Purification and characterization of soluble CD21 from human plasma by affinity chromatography and density gradient centrifugation

• Published in: Journal of immunological methods Vol. 270; no. 1; pp. 11 - 18
• Main Authors: Masilamani, Madhan, Apell, Hans-Jürgen, Illges, Harald
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.2002; Elsevier
• Subjects: Analysis of the immune response. Humoral and cellular immunity; Biological and medical sciences; Biotechnology; Cell Line; Centrifugation, Density Gradient - methods; Chromatography, Affinity - methods; Electrophoresis, Polyacrylamide Gel - methods; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Miscellaneous; Protein purification; Receptors, Complement 3d - blood; Regulatory factors and their cellular receptors; S-value; Solubility; Soluble CD21; S-value; Protein purification; Soluble CD21; Human; Molecular form; Shape; Purification; Healthy subject; Complement; Soluble form; Sedimentation coefficient; Blood plasma; Immunological method; Specific volume; Density gradient centrifugation; Cell line; Affinity chromatography; Friction coefficient; Diffusion coefficient; Molecular mass; Structural analysis; Biological receptor
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/S0022-1759(02)00211-9

Complement receptor II (CD21) is the receptor for C3d fragments on immune complexes. It also serves as a receptor for Epstein–Barr virus (EBV) and on B-lymphocytes CD21 amplifies signalling through the B cell receptor. CD21 is shed from the surface of the cell and is found circulating in plasma. The...