Immunological characterization and comparison of children with COVID-19 from their adult counterparts at single-cell resolution

• Published in: Frontiers in immunology Vol. 15; p. 1358725
• Main Authors: Jia, Ran, Li, Zifeng, Hu, Shiwen, Chang, Hailing, Zeng, Mei, Liu, Pengcheng, Lu, Lijuan, Xu, Menghua, Zhai, Xiaowen, Qian, Maoxiang, Xu, Jin
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 01.08.2024
• Subjects: Adaptive Immunity; Adolescent; Adult; Cellular Senescence - immunology; Child; Child, Preschool; children; COVID-19; COVID-19 - immunology; Cytotoxicity, Immunologic; Female; Humans; Immunology; innate immunity; Interferon Type I - immunology; Killer Cells, Natural - immunology; Male; Middle Aged; Monocytes - immunology; Monocytes - metabolism; NK cell; SARS-CoV-2 - immunology; Single-Cell Analysis; single-cell RNA sequencing; Young Adult; COVID-19; single-cell RNA sequencing; innate immunity; children; NK cell
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1358725

The immunological characteristics that could protect children with coronavirus disease 2019 (COVID-19) from severe or fatal illnesses have not been fully understood yet. Here, we performed single-cell RNA sequencing (scRNA-seq) analysis on peripheral blood samples of 15 children (8 with COVID-19) and compared them to 18 adults (13 with COVID-19). The child-adult integrated single cell data indicated that children with the disease presented a restrained response to type I interferon in most of the major immune cell types, along with suppression of upstream interferon regulatory factor and toll-like receptor expression in monocytes, which was confirmed by interferon stimulation assays. Unlike adult patients, children with COVID-19 showed lower frequencies of activated proinflammatory CD14+ monocytes, possibly explaining the rareness of cytokine storm in them. Notably, natural killer (NK) cells in pediatric patients displayed potent cytotoxicity with a rich expression of cytotoxic molecules and upregulated cytotoxic pathways, whereas the cellular senescence, along with the Notch signaling pathway, was significantly downregulated in NK cells, all suggesting more robust cytotoxicity in NK cells of children than adult patients that was further confirmed by CD107a degranulation assays. Lastly, a modest adaptive immune response was evident with more naïve T cells but less activated and proliferated T cells while less naïve B cells but more activated B cells in children over adult patients. Conclusively, this preliminary study revealed distinct cell frequency and activation status of major immune cell types, particularly more robust NK cell cytotoxicity in PBMC that might help protect children from severe COVID-19.