Secondary Amine Catalysis in Enzyme Design: Broadening Protein Template Diversity through Genetic Code Expansion

• Published in: Angewandte Chemie International Edition Vol. 63; no. 22; pp. e202403098 - n/a
• Main Authors: Williams, Thomas L., Taily, Irshad M., Hatton, Lewis, Berezin, Andrey A, Wu, Yi‐Lin, Moliner, Vicent, Świderek, Katarzyna, Tsai, Yu‐Hsuan, Luk, Louis Y. P.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 27.05.2024; John Wiley and Sons Inc
• Edition: International ed. in English
• Subjects: Amines; Artificial Enzyme; Binding; Biocatalysis; Biocatalysts; Biomimetics; Catalysis; Catalytic activity; Dihydrofolate reductase; Enzymes; Fluorescence; Genetic Code; Genetic Code Expansion; Genetic diversity; Green fluorescent protein; Green Fluorescent Proteins - chemistry; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Hydrides; Lysine - analogs & derivatives; Lysine - chemistry; Lysine - metabolism; Nucleophiles; Nucleotides; Organocatalysis; Protein Engineering; Proteins; Proteolysis; Recycling programs; Reductases; Secondary Amine Catalysis; Stereoselectivity; Tetrahydrofolate Dehydrogenase - chemistry; Tetrahydrofolate Dehydrogenase - genetics; Tetrahydrofolate Dehydrogenase - metabolism; Artificial Enzyme; Genetic Code Expansion; Organocatalysis; Secondary Amine Catalysis; Protein Engineering

Secondary amines, due to their reactivity, can transform protein templates into catalytically active entities, accelerating the development of artificial enzymes. However, existing methods, predominantly reliant on modified ligands or N‐terminal prolines, impose significant limitations on template s...