Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells

The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to the stem cells or are linked to other components of the adipose tissue. This work is aimed at analysis of the histologic and ultrastructural changes of aged facial...

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Published inPlastic and reconstructive surgery (1963) Vol. 135; no. 4; p. 999
Main Authors Charles-de-Sá, Luiz, Gontijo-de-Amorim, Natale Ferreira, Maeda Takiya, Christina, Borojevic, Radovan, Benati, Donatella, Bernardi, Paolo, Sbarbati, Andrea, Rigotti, Gino
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LanguageEnglish
Published United States 01.04.2015
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Abstract The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to the stem cells or are linked to other components of the adipose tissue. This work is aimed at analysis of the histologic and ultrastructural changes of aged facial skin after injection of fat graft in addition to its stromal vascular fraction, obtained by centrifugation, and to compare the results with those obtained by the injection of expanded adipose-derived mesenchymal stem cells. This study was performed in six consecutive patients who were candidates for face lift and whose ages ranged between 45 and 65 years. The patients underwent sampling of fat by liposuction from the abdominal region. The injection of fat and its stromal vascular fraction or expanded mesenchymal stem cells was performed in the preauricular areas. Fragments of skin were removed before and 3 months after each treatment and analyzed by optical and electron microscopy. After treatment with the autologous lipidic component and stromal vascular fraction, the skin showed a decrease in elastic fiber network (elastosis) and the appearance of new oxytalan elastic fibers in papillary dermis. The ultrastructural examination showed a modified tridimensional architecture of the reticular dermis and the presence of a richer microvascular bed. Similar results following treatment with expanded mesenchymal stem cells were observed. This study demonstrates that treatment with either fat and stromal vascular fraction or expanded mesenchymal stem cells modifies the pattern of the dermis, representing a skin rejuvenation effect.
AbstractList The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to the stem cells or are linked to other components of the adipose tissue. This work is aimed at analysis of the histologic and ultrastructural changes of aged facial skin after injection of fat graft in addition to its stromal vascular fraction, obtained by centrifugation, and to compare the results with those obtained by the injection of expanded adipose-derived mesenchymal stem cells. This study was performed in six consecutive patients who were candidates for face lift and whose ages ranged between 45 and 65 years. The patients underwent sampling of fat by liposuction from the abdominal region. The injection of fat and its stromal vascular fraction or expanded mesenchymal stem cells was performed in the preauricular areas. Fragments of skin were removed before and 3 months after each treatment and analyzed by optical and electron microscopy. After treatment with the autologous lipidic component and stromal vascular fraction, the skin showed a decrease in elastic fiber network (elastosis) and the appearance of new oxytalan elastic fibers in papillary dermis. The ultrastructural examination showed a modified tridimensional architecture of the reticular dermis and the presence of a richer microvascular bed. Similar results following treatment with expanded mesenchymal stem cells were observed. This study demonstrates that treatment with either fat and stromal vascular fraction or expanded mesenchymal stem cells modifies the pattern of the dermis, representing a skin rejuvenation effect.
Author Borojevic, Radovan
Rigotti, Gino
Gontijo-de-Amorim, Natale Ferreira
Benati, Donatella
Maeda Takiya, Christina
Charles-de-Sá, Luiz
Bernardi, Paolo
Sbarbati, Andrea
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  givenname: Luiz
  surname: Charles-de-Sá
  fullname: Charles-de-Sá, Luiz
  organization: Verona, Italy; and Rio de Janeiro, Brazil From the Department of Neurological and Motor Science, Section of Anatomy and Histology, University of Verona; the Unità di Chirurgia Rigenerativa, Clinica San Francesco; and the Postgraduate Program in Surgical Science, Federal University of Rio de Janeiro
– sequence: 2
  givenname: Natale Ferreira
  surname: Gontijo-de-Amorim
  fullname: Gontijo-de-Amorim, Natale Ferreira
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  givenname: Christina
  surname: Maeda Takiya
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  givenname: Radovan
  surname: Borojevic
  fullname: Borojevic, Radovan
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  givenname: Donatella
  surname: Benati
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  surname: Sbarbati
  fullname: Sbarbati, Andrea
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  givenname: Gino
  surname: Rigotti
  fullname: Rigotti, Gino
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Snippet The regenerative property of fat grafting has been described. However, it is not clear whether the clinical results are attributable to the stem cells or are...
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StartPage 999
SubjectTerms Adipose Tissue - cytology
Adipose Tissue - transplantation
Aged
Cosmetic Techniques
Female
Humans
Male
Mesenchymal Stem Cell Transplantation
Middle Aged
Rejuvenation
Skin Aging
Title Antiaging treatment of the facial skin by fat graft and adipose-derived stem cells
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