Development of Super-CHO protein-free medium based on a statistical design

• Published in: Journal of chemical technology and biotechnology (1986) Vol. 82; no. 5; pp. 431 - 441
• Main Authors: Huang, Edwin P, Marquis, Christopher P, Gray, Peter P
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01.05.2007; Wiley
• Subjects: Applied sciences; cell culture; Chemical engineering; CHO; Exact sciences and technology; metallothionein; promoter; protein-free; statistical design; Design; Promoter; Additive; statistical design; Experimental design; Toxicity; cell culture; protein-free; CHO; metallothionein; Optimization; Heavy metal

Based on a Plackett–Burman (P‐B) experimental design, a chemically‐defined and protein‐free medium, named Super‐CHO protein‐free (SPF), was developed to support an autocrine Super‐CHO cell line, C2.8‐SPF, to grow as a single‐cell suspension culture with a growth rate and viable cell number profile c...