FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation
FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modifica...
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| Published in | iScience Vol. 9; pp. 399 - 411 |
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| Main Authors | , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Elsevier
30.11.2018
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| Abstract | FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm. |
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| AbstractList | FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm. FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm.FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2'O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2'O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm. FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2’O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2’O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm. : Molecular Interaction; Stem Cells Research; Omics Subject Areas: Molecular Interaction, Stem Cells Research, Omics FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific set of C/D box snoRNAs in the nucleus. C/D box snoRNAs guide 2’O methylations of ribosomal RNA (rRNA) on defined sites, and this modification regulates rRNA folding and assembly of ribosomes. 2’O methylation of rRNA is partial on several sites in human embryonic stem cells, which results in ribosomes with differential methylation patterns. FMRP-snoRNA interaction affects rRNA methylation on several of these sites, and in the absence of FMRP, differential methylation pattern of rRNA is significantly altered. We found that FMRP recognizes ribosomes carrying specific methylation patterns on rRNA and the recognition of methylation pattern by FMRP may potentially determine the translation status of its target mRNAs. Thus, FMRP integrates its function in the nucleus and in the cytoplasm. • FMRP binds to C/D Box snoRNAs in the nucleus • Differential 2’O-methylation on rRNA contributes to ribosome heterogeneity in a cell • 2’O-Methylation pattern on ribosomal RNA is altered in the absence of FMRP • FMRP recognizes 2’O-methylation on rRNA, which may determine interaction with ribosomes Molecular Interaction; Stem Cells Research; Omics |
| Author | Singh, Randhir Tiwari, Vishal Selvaraj, Bhuvaneish Ramesh, Arati Chattarji, Sumantra D'Souza, Michelle Ninochka Gowda, Naveen Kumar Chandappa Anand, Praveen Dastidar, Sudhriti Ghosh Palakodeti, Dasaradhi Gulyani, Akash Pal, Rakhi Muddashetty, Ravi S. Babu, Rosana Ottakandathil James, Owen G. Chandran, Siddharthan |
| AuthorAffiliation | 4 The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India 1 Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru 560065, India 2 National Centre for Biological Sciences, Bengaluru, Karnataka 560065, India 5 Manipal Academy of Higher Education, Madhav Nagar, Manipal, Karnataka 576104, India 3 Centre for Neuroregeneration, University of Edinburgh, Edinburgh EH16 4SB, UK |
| AuthorAffiliation_xml | – name: 4 The University of Trans-Disciplinary Health Sciences & Technology (TDU), Bengaluru, Karnataka 560064, India – name: 2 National Centre for Biological Sciences, Bengaluru, Karnataka 560065, India – name: 3 Centre for Neuroregeneration, University of Edinburgh, Edinburgh EH16 4SB, UK – name: 1 Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru 560065, India – name: 5 Manipal Academy of Higher Education, Madhav Nagar, Manipal, Karnataka 576104, India |
| Author_xml | – sequence: 1 givenname: Michelle Ninochka surname: D'Souza fullname: D'Souza, Michelle Ninochka – sequence: 2 givenname: Naveen Kumar Chandappa surname: Gowda fullname: Gowda, Naveen Kumar Chandappa – sequence: 3 givenname: Vishal surname: Tiwari fullname: Tiwari, Vishal – sequence: 4 givenname: Rosana Ottakandathil surname: Babu fullname: Babu, Rosana Ottakandathil – sequence: 5 givenname: Praveen surname: Anand fullname: Anand, Praveen – sequence: 6 givenname: Sudhriti Ghosh surname: Dastidar fullname: Dastidar, Sudhriti Ghosh – sequence: 7 givenname: Randhir surname: Singh fullname: Singh, Randhir – sequence: 8 givenname: Owen G. surname: James fullname: James, Owen G. – sequence: 9 givenname: Bhuvaneish surname: Selvaraj fullname: Selvaraj, Bhuvaneish – sequence: 10 givenname: Rakhi surname: Pal fullname: Pal, Rakhi – sequence: 11 givenname: Arati surname: Ramesh fullname: Ramesh, Arati – sequence: 12 givenname: Sumantra surname: Chattarji fullname: Chattarji, Sumantra – sequence: 13 givenname: Siddharthan surname: Chandran fullname: Chandran, Siddharthan – sequence: 14 givenname: Akash surname: Gulyani fullname: Gulyani, Akash – sequence: 15 givenname: Dasaradhi surname: Palakodeti fullname: Palakodeti, Dasaradhi – sequence: 16 givenname: Ravi S. surname: Muddashetty fullname: Muddashetty, Ravi S. |
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| Snippet | FMRP is an RNA-binding protein that is known to localize in the cytoplasm and in the nucleus. Here, we have identified an interaction of FMRP with a specific... |
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| Title | FMRP Interacts with C/D Box snoRNA in the Nucleus and Regulates Ribosomal RNA Methylation |
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