Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men： a preliminary investigation

• Published in: Asian journal of andrology Vol. 14; no. 4; pp. 566 - 573
• Main Authors: Hou, Dong-Sheng, Long, Wen-Min, Shen, Jian, Zhao, Li-Ping, Pang, Xiao-Yan, Xu, Chen
• Format: Journal Article
• Language: English
• Published: China Medknow Publications & Media Pvt. Ltd 01.07.2012; Nature Publishing Group
• Subjects: Adult; Bacillaceae - isolation & purification; Bacteria; Bodily Secretions - chemistry; Bodily Secretions - microbiology; Caulobacteraceae - isolation & purification; Chronic Disease; Community structure; DNA; Ecology; Gel electrophoresis; Humans; Infertility; Infertility, Male - microbiology; Least-Squares Analysis; Male; Microenvironments; Original; Pain; Pelvis; Primers; Principal Component Analysis; Principal components analysis; Prostate; Prostate - microbiology; Prostate - secretion; Prostatitis; Prostatitis - microbiology; Reproductive system; RNA, Bacterial - analysis; RNA, Ribosomal, 16S - analysis; rRNA 16S; Secretions; Urethra; Young Adult; 分泌物; 患者; 慢性前列腺炎; 特征描述; 男性不育症; 疼痛; 细菌群落; 综合征
• ISSN: 1008-682X; 1745-7262; 1745-7262
• DOI: 10.1038/aja.2012.30

The expressed prostatic secretions （EPSs） of men with chronic prostatitis/chronic pelvic pain syndrome （CP/CPPS）, infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis （DGGE） method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis （PCA） and partial least squares （PLS） analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients （P〈O.05）. Two bands were identified as priority factors in the discrepancy of category IliA and category IIIB prostatitis patients （P〈O.05）. According to this research, the ecological balance of the prostate and low urethra tract, when considered as a microenvironment, might play an important role in the maintenance of a healthy male reproductive tract.