A majority of Ig H chain cDNA of normal human adult blood lymphocytes resembles cDNA for fetal Ig and natural autoantibodies

• Published in: The Journal of immunology (1950) Vol. 151; no. 10; pp. 5290 - 5300
• Main Authors: Huang, C, Stollar, BD
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 15.11.1993; American Association of Immunologists
• Subjects: Adult; ANIMAL CELLS; Autoantibodies - genetics; Base Sequence; BASIC BIOLOGICAL SCIENCES; Biological and medical sciences; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; DNA SEQUENCING; DNA, Complementary - analysis; DNA, Complementary - chemistry; Female; Fetus - immunology; Fundamental and applied biological sciences. Psychology; GENES; Genes, Immunoglobulin; Genes. Genome; GLOBULINS; Humans; Immunoglobulin G - genetics; Immunoglobulin Heavy Chains - genetics; Immunoglobulin M - genetics; Immunoglobulin Variable Region - genetics; IMMUNOGLOBULINS; LEUKOCYTES; LYMPHOCYTES; Lymphocytes - immunology; MATERIALS; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutation; NEOPLASMS; ORGANIC COMPOUNDS; Pregnancy; PROTEINS; SOMATIC CELLS; STRUCTURAL CHEMICAL ANALYSIS 550400 > Genetics; Human; Heavy peptide chain; Immunoglobulins; Complementary DNA; Variable region; Autoantibody; Adult; Fetus; B-Lymphocyte; Mutation; Gene expression

Certain Ig VH gene segments, with few or no mutations, recur frequently in natural autoantibodies, fetal antibodies, and products of B cell tumors. The goal of this study was to determine whether similar Ig gene segment usage occurs in normal human adult PBL. Extending previous analyses, 105 randoml...