Agrobacterium-Mediated Transformation in Alpinia galanga (Linn.) Willd. for Enhanced Acetoxychavicol Acetate Production

• Published in: Applied biochemistry and biotechnology Vol. 168; no. 2; pp. 339 - 347
• Main Authors: Rao, Kiranmayee, Chodisetti, Bhuvaneswari, Mangamoori, Lakshmi Narasu, Giri, Archana
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.09.2012; Springer; Springer Nature B.V
• Subjects: acetates; Agrobacterium - genetics; Agrobacterium rhizogenes; Alpinia - genetics; Alpinia - growth & development; Alpinia - metabolism; Alpinia galanga; Bacteriology; Benzyl Alcohols - metabolism; Bioactive compounds; Biochemistry; Biological and medical sciences; Biotechnology; Chemistry; Chemistry and Materials Science; Fundamental and applied biological sciences. Psychology; Genetic Engineering - methods; genetic stability; Genetics; high performance liquid chromatography; HIV; Human immunodeficiency virus; Liquid chromatography; Metabolites; Pharmacology; Plant growth; Rhizobium rhizogenes; Roots; Secondary metabolites; Transformation, Genetic; Hairy roots; Acetoxychavicol acetate; HPLC; Hairy root; Genetic transformation; Production; HPLC chromatography; Bacteria; Rhizobiaceae; Acetate; Agrobacterium rhizogenes; Alpinia galanga
• DOI: 10.1007/s12010-012-9777-6
• ISSN: 0273-2289; 1559-0291

Agrobacterium -mediated transformations ensure elevated amounts of secondary metabolite accumulation with genetic and biosynthetic stability. In the present study, Alpinia galanga rich in bioactive compounds was genetically transformed using different strains of Agrobacterium rhizogenes viz. LBA 9402 , A 4 , 532 , 2364 and PRT Gus . Even though a higher growth rate was obtained with the LBA 9402 strain, maximum acetoxychavicol acetate accumulation (ACA) was seen in the PRT Gus transformant. PRT Gus root line has shown 10.1 fold higher ACA content in comparison to the control roots. The lowest ACA production was shown by the A 4 transformant (4.9 fold). The quantification of ACA in the transformed roots was carried out by using HPLC, which was found to be in the order of PRT Gus > LBA 9402 > 2364 > 532 > A 4 . The fast growth rate of hairy roots, genetic stability and their ability to synthesize more than one metabolite offer a promising system for the production of valuable secondary metabolites.