Morphological changes during replicative senescence in bovine ovarian granulosa cells

• Published in: Cell cycle (Georgetown, Tex.) Vol. 18; no. 13; pp. 1490 - 1497
• Main Authors: Son, Hoang Nghia, Chi, Ho Nguyen Quynh, Chung, Doan Chinh, Long, Le Thanh
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03.07.2019
• Subjects: Bovine granulosa cells; long-term culture; morphology; replicative senescence; Research Paper; long-term culture; replicative senescence; morphology; Bovine granulosa cells

The objective of this study was to evaluate replicative senescence of bovine granulosa cells (bGCs) during in vitro long-term culture. WST-1 assay analysis showed that bGCs proliferation was reduced from primary culture to 14th passage. The several bGCs from the 3rd passage and 7th passage exposed t...