Shoot regeneration from cultured root explants of spinach (Spinacia oleracea L.): a system for Agrobacterium transformation

• Published in: Plant cell reports Vol. 17; no. 2; pp. 96 - 101
• Main Authors: Knoll, K.A, Short, K.C, Curtis, I.S, Power, J.B, Davey, M.R
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.12.1997; Springer Nature B.V
• Subjects: Acids; Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; beta-glucuronidase; Biological and medical sciences; Biotechnology; culture media; developmental stages; Eukaryotic cell cultures; explants; Fundamental and applied biological sciences. Psychology; gene transfer; genes; genetic transformation; Genetics and breeding of economic plants; gibberellic acid; Grasses; Haploidy, in vitro culture applications, somatic hybrids; in vitro culture; In vitro propagation: entire plant regeneration from tissues and cell cultures; indole acetic acid; Methods. Procedures. Technologies; micropropagation; murashige and skoog medium; naphthaleneacetic acid; nitsch and nitsch medium; plant anatomy; Plant breeding: fundamental aspects and methodology; Plant cells and fungal cells; plant morphology; regenerative ability; roots; shoots; Spinacia oleracea; Auxin; 1-Naphthaleneacetic acid; Tissue culture; Explant; Spinacia oleracea; Agrobacterium; Dicotyledones; Indoleacetic acid; Angiospermae; Bacteria; Plant growth substance; Culture medium; Vegetals; β-Glucuronidase; Genetic transformation; Root; Enzyme; Gibberellin; Gene expression; Chenopodiaceae; Regeneration; Vegetable crop; Glycosidases; Hydrolases; Spermatophyta; Rhizobiaceae; Gibberellic acid; O-Glycosidases; Transformation; Shoot regeneration; Key words Spinach; Roots
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s002990050359

A reliable plant regeneration system is described for the production of adventitious shoots from root explants of spinach. Explants from roots of axenic shoots and roots induced on cultured hypocotyl explants were used for adventitious shoot induction. Explants from apical, middle and basal root regions were incubated on Nitsch and Nitsch medium supplemented with alpha-naphthaleneacetic acid, gibberellic acid and kinetin. Optimum shoot regeneration was from explants of apical and middle root regions on medium with 20 micromolar alpha-naphthaleneacetic acid and 5.0 micromolar gibberellic acid. Shoots originated directly from root tissues without an intervening callus phase. Adventitious shoots were rooted and were grown to maturity in the glasshouse. This plant regeneration procedure has been exploited in preliminary studies of Agrobacterium-mediated transformation.