Evidence that Sp1 positively and Sp3 negatively regulate and androgen does not directly regulate functional tumor suppressor 15-lipoxygenase 2 (15-LOX2) gene expression in normal human prostate epithelial cells

• Published in: Oncogene Vol. 23; no. 41; pp. 6942 - 6953
• Main Authors: SHAOHUA TANG, BHATIA, Bobby, JIANJUN ZHOU, MALDONADO, Carlos J, CHANDRA, Dhyan, KIM, Eunjung, FISCHER, Susan M, BUTLER, Andrew P, FRIEDMAN, Scott L, TANG, Dean G
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 09.09.2004; Nature Publishing Group
• Subjects: Androgen receptors; Androgens; Androgens - physiology; Arachidonate 15-Lipoxygenase - genetics; Base Sequence; Biological and medical sciences; Boxes; Cell physiology; Cell transformation and carcinogenesis. Action of oncogenes and antioncogenes; DNA-Binding Proteins - physiology; Electrophoretic mobility; Epithelial cells; Epithelial Cells - enzymology; Fundamental and applied biological sciences. Psychology; Gene deletion; Gene expression; Gene Expression Regulation, Enzymologic; Gene regulation; Gynecology. Andrology. Obstetrics; Humans; Lipoxygenase; LOX2 gene; Male; Male genital diseases; Medical sciences; Molecular and cellular biology; Molecular Sequence Data; Nephrology. Urinary tract diseases; Promoter Regions, Genetic; Prostate; Prostate - enzymology; Prostate cancer; Sp1 protein; Sp1 Transcription Factor - physiology; Sp3 Transcription Factor; Transcription factors; Transcription Factors - physiology; Tumor suppressor genes; Tumors; Tumors of the urinary system; Urinary tract. Prostate gland; Human; Urinary system disease; Prostate disease; Androgen; Enzyme; Malignant tumor; Gene expression; Normal; Carcinogenesis; Regulation(control); 15-lipoxygenase 2; Sp1; gene regulation; Sp3; Epithelial cell; Oxidoreductases; Sex steroid hormone; Transcription factor; Lipoxygenase; Male genital diseases; Urogenital system; Prostate cancer; Tumor suppressor gene

In this project, we studied the gene regulation of 15-lipoxygenase 2 (15-LOX2), the most abundant arachidonate-metabolizing LOX in adult human prostate and a negative cell-cycle regulator in normal human prostate (NHP) epithelial cells. Through detailed in silico promoter examination and promoter de...