Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins

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Published inJournal of Virology Vol. 69; no. 2; pp. 1013 - 1018
Main Authors Dragic, T, Picard, L, Alizon, M
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 01.02.1995
Subjects
3T3 Cells
AIDS/HIV
Animals
CD4 Antigens - physiology
Cell Line
Endopeptidase K
Erythrocyte Membrane - physiology
Gene Products, env - physiology
HIV-1 - physiology
human immunodeficiency virus 1
Humans
Leukemia, Erythroblastic, Acute - pathology
Membrane Fusion
Mice
Pronase - pharmacology
Serine Endopeptidases - pharmacology
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Abstract Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue JVI About JVI Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy JVI RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0022-538X Online ISSN: 1098-5514 Copyright © 2014 by the American Society for Microbiology.   For an alternate route to JVI .asm.org, visit: JVI       
AbstractList Murine CD4+ cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins (Env). The role of human-specific factors in Env/CD4-mediated fusion is shown by the ability of transient cell hybrids formed between CD4+ murine cells and human HeLa cells to fuse with Env+ cells. Fusion events were observed when other human cells, including erythrocytes, were substituted for HeLa cells in the hybrids. Experiments with erythrocyte ghosts showed that the factors allowing Env/CD4-mediated fusion are located in the plasma membrane. These factors were fully active after extensive digestion of erythrocytes with proteinase K or pronase. Nonprotein components of human plasma membranes, possibly glycolipids, could therefore be required for Env/CD4-mediated fusion and virus entry.
Murine CD4 super(+) cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins (Env). The role of human-specific factors in Env/CD4-mediated fusion is shown by the ability of transient cell hybrids formed between CD4 super(+) murine cells and human HeLa cells to fuse with Env super(+) cells. Fusion events were observed when other human cells, including erythrocytes, were substituted for HeLa cells in the hybrids. Experiments with erythrocyte ghosts showed that the factors allowing Env/CD4-mediated fusion are located in the plasma membrane. These factors were fully active after extensive digestion of erythrocytes with proteinase K or pronase. Nonprotein components of human plasma membranes, possibly glycolipids, could therefore be required for Env/CD4-mediated fusion and virus entry.
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Author T Dragic
M Alizon
L Picard
AuthorAffiliation Institut National de la Santé et de la Recherche Médicale U332, Institut Cochin de Génétique Moléculaire, Paris, France
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Snippet Article Usage Stats Services JVI Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley...
Murine CD4+ cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins (Env)....
Murine CD4 super(+) cells are resistant to human immunodeficiency virus type 1 (HIV-1) entry and to fusion with cells expressing HIV-1 envelope glycoproteins...
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SubjectTerms 3T3 Cells
AIDS/HIV
Animals
CD4 Antigens - physiology
Cell Line
Endopeptidase K
Erythrocyte Membrane - physiology
Gene Products, env - physiology
HIV-1 - physiology
human immunodeficiency virus 1
Humans
Leukemia, Erythroblastic, Acute - pathology
Membrane Fusion
Mice
Pronase - pharmacology
Serine Endopeptidases - pharmacology
Title Proteinase-resistant factors in human erythrocyte membranes mediate CD4-dependent fusion with cells expressing human immunodeficiency virus type 1 envelope glycoproteins
URI http://jvi.asm.org/content/69/2/1013.abstract
https://www.ncbi.nlm.nih.gov/pubmed/7815477
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Volume 69
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