RNA secondary structure at the transcription start site influences EBOV transcription initiation and replication in a length- and stability-dependent manner

• Published in: RNA biology Vol. 18; no. 4; pp. 523 - 536
• Main Authors: Bach, Simone, Demper, Jana-Christin, Biedenkopf, Nadine, Becker, Stephan, Hartmann, Roland K.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03.04.2021
• Subjects: EBOV 3ʹ-leader promoter; expansion of the spacer between PE1 and PE2; Research Paper; RNA stabilization of hairpin structures at the transcription start site (TSS); Viral transcription and replication; Viral transcription and replication; expansion of the spacer between PE1 and PE2; RNA stabilization of hairpin structures at the transcription start site (TSS); EBOV 3ʹ-leader promoter

Ebola virus (EBOV) RNA has the potential to form hairpin structures at the transcription start sequence (TSS) and reinitiation sites of internal genes, both on the genomic and antigenomic/mRNA level. Hairpin formation involving the TSS and the spacer sequence between promotor elements (PE) 1 and 2 w...