A standardized immune phenotyping and automated data analysis platform for multicenter biomarker studies

• Published in: JCI insight Vol. 3; no. 23
• Main Authors: Ivison, Sabine, Malek, Mehrnoush, Garcia, Rosa V., Broady, Raewyn, Halpin, Anne, Richaud, Manon, Brant, Rollin F., Wang, Szu-I, Goupil, Mathieu, Guan, Qingdong, Ashton, Peter, Warren, Jason, Rajab, Amr, Urschel, Simon, Kumar, Deepali, Streitz, Mathias, Sawitzki, Birgit, Schlickeiser, Stephan, Bijl, Janetta J., Wall, Donna A., Delisle, Jean-Sebastien, West, Lori J., Brinkman, Ryan R., Levings, Megan K.
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 06.12.2018
• Subjects: Adaptive Immunity; Biomarkers - blood; Cryopreservation - methods; Cryopreservation - standards; Data Analysis; Electronic Data Processing; Flow Cytometry - methods; Flow Cytometry - standards; Hematopoietic Stem Cell Transplantation; Humans; Immunity, Innate; Immunophenotyping - methods; Immunophenotyping - standards; L-Selectin; Leukocyte Common Antigens; Leukocytes, Mononuclear - immunology; Monocytes; Reproducibility of Results; Stem cell transplantation; Innate immunity; Adaptive immunity; Transplantation; Immunology

The analysis and validation of flow cytometry-based biomarkers in clinical studies are limited by the lack of standardized protocols that are reproducible across multiple centers and suitable for use with either unfractionated blood or cryopreserved PBMCs. Here we report the development of a platfor...