Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli

• Published in: iScience Vol. 14; pp. 323 - 334
• Main Authors: Fedorec, Alex J.H., Ozdemir, Tanel, Doshi, Anjali, Ho, Yan-Kay, Rosa, Luca, Rutter, Jack, Velazquez, Oscar, Pinheiro, Vitor B., Danino, Tal, Barnes, Chris P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier 26.04.2019
• Subjects: Bioengineering; Biological Sciences; Gene Network

Plasmids are the workhorse of both industrial biotechnology and synthetic biology, but ensuring they remain in bacterial cells is a challenge. Antibiotic selection cannot be used to stabilize plasmids in most real-world applications, and inserting dynamical gene networks into the genome remains chal...