Metatranscriptomics of Nasopharyngeal Microbiota and Host Distinguish between Pneumonia and Health

• Published in: American journal of respiratory and critical care medicine Vol. 206; no. 12; pp. 1564 - 1567
• Main Authors: Nannapaneni, Priyanka, Sundh, John, Prast-Nielsen, Stefanie, Rhedin, Samuel, Örtqvist, Åke, Naucler, Pontus, Henriques-Normark, Birgitta
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 15.12.2022
• Subjects: Antibiotics; Children & youth; Correspondence; Humans; Microbiota; Microbiota - genetics; Nasopharynx; Nose; Nutrition; Otolaryngology; Pediatrics; Pneumonia; RNA, Ribosomal, 16S; Throat

In order to map the expression of the nasopharyngeal microbiota and associated host responses in 20 healthy children and 20 children with CAP, we developed a novel metatranscriptomic pipeline with ultrasensitive sequencing of nasopharyngeal aspirates (https://doi.org/10.6084/m9.figshare.20452026.v2)...