Structural investigation of cellobiose dehydrogenase IIA: Insights from small angle scattering into intra- and intermolecular electron transfer mechanisms

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 4; pp. 1031 - 1039
• Main Authors: Bodenheimer, Annette M., O'Dell, William B., Oliver, Ryan C., Qian, Shuo, Stanley, Christopher B., Meilleur, Flora
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2018; Elsevier
• Subjects: Ascomycota - enzymology; Ascomycota - genetics; BASIC BIOLOGICAL SCIENCES; calcium; Calcium - chemistry; Calcium - metabolism; carbohydrate binding; Carbohydrate Dehydrogenases - chemistry; Carbohydrate Dehydrogenases - genetics; Carbohydrate Dehydrogenases - metabolism; cations; cellobiose; cellobiose dehydrogenase; Cellulose - chemistry; Cellulose - metabolism; electron transfer; Electron Transport; fuel production; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Hydrogen-Ion Concentration; Intermolecular electron transfer (IeET); Intramolecular electron transfer (IaET); medical equipment; Modeling; Models, Molecular; Neurospora crassa; Neurospora crassa - enzymology; Neurospora crassa - genetics; neutrons; oxidation; Oxidation-Reduction; Oxidative cellulose degradation; PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; polysaccharides; Protein Binding; Protein Conformation; Redox complex; Scattering, Small Angle; Small-angle scattering; X-radiation; X-Ray Diffraction; Intermolecular electron transfer (IeET); Small-angle scattering; Oxidative cellulose degradation; Intramolecular electron transfer (IaET); Modeling; Redox complex

Cellobiose dehydrogenases have gained interest due to their potential applications in sectors from biofuel production to biomedical devices. The CDHIIA variant is comprised of a cytochrome domain (CYT), a dehydrogenase domain (DH), and a carbohydrate-binding module (CBM) that are connected by two fl...