Detection of mutant KRAS and TP53 DNA in circulating exosomes from healthy individuals and patients with pancreatic cancer

• Published in: Cancer biology & therapy Vol. 18; no. 3; pp. 158 - 165
• Main Authors: Yang, Sujuan, Che, Sara P. Y., Kurywchak, Paul, Tavormina, Jena L., Gansmo, Liv B., Correa de Sampaio, Pedro, Tachezy, Michael, Bockhorn, Maximilian, Gebauer, Florian, Haltom, Amanda R., Melo, Sonia A., LeBleu, Valerie S., Kalluri, Raghu
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 04.03.2017
• Subjects: Case-Control Studies; Cell Line, Tumor; Circulating exosomal DNA; digital PCR; DNA - blood; DNA - genetics; DNA, Neoplasm - blood; DNA, Neoplasm - genetics; exosome; Exosomes - genetics; Humans; KRAS; liquid biopsy; Mutation; pancreatic cancer; Pancreatic Neoplasms - blood; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; Polymerase Chain Reaction - methods; Proto-Oncogene Proteins p21(ras) - genetics; Research Paper; TP53; Tumor Suppressor Protein p53 - genetics; pancreatic cancer; Circulating exosomal DNA; digital PCR; KRAS; TP53; exosome; liquid biopsy

Pancreatic cancer presents with a dismal mortality rate and is in urgent need of methods for early detection with potential for timely intervention. All living cells, including cancer cells, generate exosomes. We previously discovered double stranded genomic DNA in exosomes derived from the circulat...