Rainbow-Seq: Combining Cell Lineage Tracing with Single-Cell RNA Sequencing in Preimplantation Embryos

• Published in: iScience Vol. 7; pp. 16 - 29
• Main Authors: Biase, Fernando H., Wu, Qiuyang, Calandrelli, Riccardo, Rivas-Astroza, Marcelo, Zhou, Shuigeng, Chen, Zhen, Zhong, Sheng
• Format: Journal Article
• Language: English
• Published: United States Elsevier 28.09.2018
• Subjects: Single cell; Preimplantation; Cell fate; Lineage tracing; Transposon

We developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers, Rainbow-seq enables each single-cell RNA sequencing (RNA-seq) experiment to simultaneously decode the lineage marker genes and rea...