H/D exchange mass spectrometry and statistical coupling analysis reveal a role for allostery in a ferredoxin-dependent bifurcating transhydrogenase catalytic cycle

• Published in: Biochimica et biophysica acta. General subjects Vol. 1862; no. 1; pp. 9 - 17
• Main Authors: Berry, Luke, Poudel, Saroj, Tokmina-Lukaszewska, Monika, Colman, Daniel R., Nguyen, Diep M.N., Schut, Gerrit J., Adams, Michael W.W., Peters, John W., Boyd, Eric S., Bothner, Brian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.01.2018; Elsevier
• Subjects: Allosteric Regulation; Allostery; Archaeal Proteins - chemistry; Archaeal Proteins - metabolism; BASIC BIOLOGICAL SCIENCES; binding sites; bio-inspired; biofuels (including algae and biomass); catalytic activity; deuterium; Deuterium Exchange Measurement - methods; Electron bifurcation; electron transfer; Ferredoxin-dependent transhydrogenase; ferredoxin-NADP reductase; Ferredoxins - chemistry; Ferredoxins - metabolism; Flavin-based electron bifurcation; HDX-MS; hydrogen and fuel cells; ligands; mass spectrometry; metabolism; NADP (coenzyme); NADP Transhydrogenases - chemistry; NADP Transhydrogenases - metabolism; oxidation; protein subunits; proteins; Pyrococcus furiosus - enzymology; SCA; solar (fuels); spectral analysis; Flavin-based electron bifurcation; D; NAD+/NADH; Electron bifurcation; FdOx; Nfn-S; FAD; Ferredoxin-dependent transhydrogenase; Allostery; Nfn-L; NADP+/NADPH; SCA; HDX-MS; Pf; Nfn; FBEB; Tm; FdRed

Recent investigations into ferredoxin-dependent transhydrogenases, a class of enzymes responsible for electron transport, have highlighted the biological importance of flavin-based electron bifurcation (FBEB). FBEB generates biomolecules with very low reduction potential by coupling the oxidation of...