45-KDa GTP-binding Protein from Rat Olfactory Epithelium: Purification, Characterization and Localization

• Published in: Chemical senses Vol. 21; no. 2; pp. 181 - 188
• Main Authors: Novoselov, V.I., Peshenko, I.V., Evdokimov, V.A., Nikolaev, J.V., Matveeva, E.A., Fesenko, E.E.
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 01.04.1996
• Subjects: Affinity Labels; Amino Acid Sequence; Animals; Antibodies; Epithelium - chemistry; GTP-Binding Proteins - chemistry; GTP-Binding Proteins - immunology; GTP-Binding Proteins - isolation & purification; Guanosine Triphosphate - chemistry; Immunoblotting; Male; Molecular Sequence Data; Molecular Weight; Olfactory Mucosa - chemistry; Phosphorus Radioisotopes; Rabbits; Rats; Rats, Wistar; Turbinates
• ISSN: 0379-864X; 1464-3553
• DOI: 10.1093/chemse/21.2.181

The rat olfactory epithelium contains a specific water-soluble 45-kDa protein. This protein is recognized by anti-peptide antibodies which react with α-subunits of the known G-proteins. The 45-kDa protein has been isolated using DEAE-chromatography and gel-exclusion chromatography. The content of 45-kDa protein is about 2% of the total soluble proteins of the olfactory mucosa and it is located at the mucociliary surface. According to photo-affinity labeling, the 45-kDa protein possesses a high affinity to GTP and exhibits low GTP hydrolytic activity. The functions of the 45-kDa protein are discussed. Chem, Senses 21: 181–188, 1996.