Neurophysin-neurohypophyseal hormone interactions: studies using a dansylated vasotocin analogue

• Published in: International journal of peptide and protein research Vol. 38; no. 5; p. 459
• Main Authors: Hasselbacher, C A, Schwartz, G P, Glass, J D, Laws, W R
• Format: Journal Article
• Language: English
• Published: Denmark 01.11.1991
• Subjects: Amino Acid Sequence; Amino Acids - analysis; Animals; Cattle; Dansyl Compounds - chemistry; Dansyl Compounds - metabolism; Fluorescent Dyes; Kinetics; Molecular Sequence Data; Neurophysins - isolation & purification; Neurophysins - metabolism; Protein Binding; Spectrometry, Fluorescence; Vasotocin - analogs & derivatives; Vasotocin - chemistry; Vasotocin - metabolism

We have synthesized a neurohypophyseal hormone analogue containing an extrinsic fluorescence probe by linking a dansyl (DNS) group to the epsilon-amino group of the lysine at residue 8 of vasotocin. The fluorescence properties of this analogue have been characterized by steady-state and time-resolve...