Reprogramming of Fibroblasts From Older Women With Pelvic Floor Disorders Alters Cellular Behavior Associated With Donor Age

• Published in: Stem cells translational medicine Vol. 2; no. 2; pp. 118 - 128
• Main Authors: Wen, Yan, Wani, Prachi, Zhou, Lu, Baer, Tom, Phadnis, Smruti Madan, Reijo Pera, Renee A., Chen, Bertha
• Format: Journal Article
• Language: English
• Published: United States AlphaMed Press 01.02.2013; Oxford University Press
• Subjects: Adult Stem Cells - cytology; Adult Stem Cells - physiology; Age; Age differences; Age Factors; Aged; Aging; Animals; Cell Differentiation - physiology; Cellular Senescence - physiology; Data analysis; Embryoid Bodies - cytology; Fecal incontinence; Female; Fibroblasts; Fibroblasts - cytology; Fibroblasts - physiology; Gene expression; Genetic Vectors; Humans; Induced pluripotent stem cells; Karyotyping; Lentivirus - genetics; Mice; Middle Aged; Mitosis - physiology; Oxidative stress; p53; Pelvic Floor Disorders - pathology; Pelvic organ prolapse; Pluripotency; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - physiology; Primary Cell Culture; Reprogramming; Senescence; Smooth muscle; Stem cells; Studies; Telomerase; Teratoma - pathology; Tissue-Specific Progenitor and Stem Cells; Tumor Suppressor Protein p53 - genetics; Urinary incontinence; Vagina; Vagina - cytology; Women; Writing

The effect of donor age on induced pluripotent stem cell (iPSC) lines and on the cells redifferentiated from these iPSCs was examined. iPSCs were derived from vaginal fibroblasts from women with pelvic organ prolapse. Donor age did not appear to affect reprogramming and cell mitotic activity in fibr...