Reactive oxygen species influence nerve growth factor synthesis in primary rat astrocytes

• Published in: Journal of neurochemistry Vol. 62; no. 6; p. 2178
• Main Authors: Naveilhan, P, Neveu, I, Jehan, F, Baudet, C, Wion, D, Brachet, P
• Format: Journal Article
• Language: English
• Published: England 01.06.1994
• Subjects: Animals; Anisomycin - pharmacology; Astrocytes - metabolism; Catalase - pharmacology; Cells, Cultured; Hydrogen Peroxide - pharmacology; Nerve Growth Factors - biosynthesis; Rats; Reactive Oxygen Species - pharmacology; Xanthine; Xanthine Oxidase - pharmacology; Xanthines - pharmacology

Newborn rat brain astrocytes, cultured in a serum-free medium, were exposed for 30 min to two types of reactive oxygen species. Cells were either treated with the xanthine/xanthine oxidase (X/XOD) system, which generates both H2O2 and the O2.- radical, or to H2O2 alone. Both treatments induced a dos...