Evolution and Single‐Droplet Analysis of Fuel‐Driven Compartments by Droplet‐Based Microfluidics
Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet‐based microfluidics to encapsulate a fuel‐driven cycle that drives phase separation into coace...
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| Published in | Angewandte Chemie International Edition Vol. 61; no. 32; pp. e202203928 - n/a |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Germany
Wiley Subscription Services, Inc
08.08.2022
John Wiley and Sons Inc |
| Edition | International ed. in English |
| Subjects | |
| Online Access | Get full text |
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| Abstract | Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet‐based microfluidics to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets to overcome this challenge. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate‐based droplets and their properties. We observed that coacervate‐based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate‐based droplets enables future selection‐ or evolution‐based studies.
Droplet‐based microfluidics were used to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. The nucleation, growth, and dissolution of active coacervate‐based droplets were investigated with this setup. |
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| AbstractList | Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We use droplet-based microfluidics to encapsulate a fuel-driven cycle that drives phase separation into coacervate droplets to overcome this challenge. This approach enables the analysis of every coacervate-based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate-based droplets and their properties. We observe that coacervate-based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate-based droplets enables future selection- or evolution-based studies. Abstract Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet‐based microfluidics to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets to overcome this challenge. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate‐based droplets and their properties. We observed that coacervate‐based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate‐based droplets enables future selection‐ or evolution‐based studies. Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet‐based microfluidics to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets to overcome this challenge. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate‐based droplets and their properties. We observed that coacervate‐based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate‐based droplets enables future selection‐ or evolution‐based studies. Droplet‐based microfluidics were used to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. The nucleation, growth, and dissolution of active coacervate‐based droplets were investigated with this setup. Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short timescales of their kinetics. We used droplet‐based microfluidics to encapsulate a fuel‐driven cycle that drives phase separation into coacervate‐based droplets to overcome this challenge. This approach enables the analysis of every coacervate‐based droplet in the reaction container throughout its lifetime. We discovered that the fuel concentration dictates the formation of the coacervate‐based droplets and their properties. We observed that coacervate‐based droplets grow through fusion, decay simultaneously independent of their volume, and shrinkage rate scales with their initial volume. This method helps to further understand the regulation of membraneless organelles, and we believe the analysis of individual coacervate‐based droplets enables future selection‐ or evolution‐based studies. |
| Author | Donau, Carsten Boekhoven, Job Göpfrich, Kerstin Jahnke, Kevin Späth, Fabian Bergmann, Alexander M. |
| AuthorAffiliation | 1 Department of Chemistry Technical University of Munich Lichtenbergstrasse 4 85748 Garching Germany 3 Department of Physics and Astronomy Heidelberg University 69120 Heidelberg Germany 2 Biophysical Engineering Group Max Planck Institute for Medical Research Jahnstraße 29 69120 Heidelberg Germany |
| AuthorAffiliation_xml | – name: 2 Biophysical Engineering Group Max Planck Institute for Medical Research Jahnstraße 29 69120 Heidelberg Germany – name: 1 Department of Chemistry Technical University of Munich Lichtenbergstrasse 4 85748 Garching Germany – name: 3 Department of Physics and Astronomy Heidelberg University 69120 Heidelberg Germany |
| Author_xml | – sequence: 1 givenname: Alexander M. surname: Bergmann fullname: Bergmann, Alexander M. organization: Technical University of Munich – sequence: 2 givenname: Carsten surname: Donau fullname: Donau, Carsten organization: Technical University of Munich – sequence: 3 givenname: Fabian surname: Späth fullname: Späth, Fabian organization: Technical University of Munich – sequence: 4 givenname: Kevin surname: Jahnke fullname: Jahnke, Kevin organization: Heidelberg University – sequence: 5 givenname: Kerstin surname: Göpfrich fullname: Göpfrich, Kerstin email: kerstin.goepfrich@mr.mpg.de organization: Heidelberg University – sequence: 6 givenname: Job orcidid: 0000-0002-9126-2430 surname: Boekhoven fullname: Boekhoven, Job email: job.boekhoven@tum.de organization: Technical University of Munich |
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| CitedBy_id | crossref_primary_10_1002_ange_202211905 crossref_primary_10_1002_anie_202309318 crossref_primary_10_1021_jacs_3c03793 crossref_primary_10_1002_anie_202211905 crossref_primary_10_1016_j_matt_2023_12_025 crossref_primary_10_1002_smtd_202300496 crossref_primary_10_1002_syst_202300034 crossref_primary_10_1016_j_cclet_2024_109995 crossref_primary_10_1021_accountsmr_2c00239 crossref_primary_10_1002_ange_202309318 crossref_primary_10_1021_accountsmr_2c00244 crossref_primary_10_1016_j_nbt_2024_02_003 crossref_primary_10_1002_ange_202216537 crossref_primary_10_1002_advs_202305760 crossref_primary_10_1002_anie_202216537 crossref_primary_10_1016_j_chempr_2023_08_029 |
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| Keywords | artificial organelles, droplet-based microfluidics, nonequilibrium processes, phase transitions |
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| Snippet | Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to the short... Abstract Active droplets are a great model for membraneless organelles. However, the analysis of these systems remains challenging and is often limited due to... |
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| SubjectTerms | Artificial Organelles Communication Communications Droplet-Based Microfluidics Droplets Evolution Fuels Microfluidics Nonequilibrium Processes Organelles Phase separation Phase Transitions |
| Title | Evolution and Single‐Droplet Analysis of Fuel‐Driven Compartments by Droplet‐Based Microfluidics |
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