NADH Autofluorescence—A Marker on its Way to Boost Bioenergetic Research

• Published in: Cytometry. Part A Vol. 95; no. 1; pp. 34 - 46
• Main Authors: Schaefer, Patrick M., Kalinina, Sviatlana, Rueck, Angelika, von Arnim, Christine A.F., von Einem, Bjoern
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.01.2019; Wiley Subscription Services, Inc
• Subjects: Bioenergetics; Cytometry; Energy metabolism; Energy Metabolism - physiology; FLIM; Fluorescence; Imaging; In vivo methods and tests; Metabolism; Microscopy, Fluorescence - methods; Mitochondria; Mitochondria - metabolism; NAD - chemistry; NADH; Nicotinamide adenine dinucleotide; Optical Imaging; Oxidation-Reduction; Redox properties; Spatial discrimination; Spatial resolution; Spectrometry, Fluorescence; FLIM; metabolism; NADH; bioenergetics; imaging; mitochondria

More than 60 years ago, the idea was introduced that NADH autofluorescence could be used as a marker of cellular redox state and indirectly also of cellular energy metabolism. Fluorescence lifetime imaging microscopy of NADH autofluorescence offers a marker‐free readout of the mitochondrial function...