Long term bed rest with and without vibration exercise countermeasures: Effects on human muscle protein dysregulation

• Published in: Proteomics (Weinheim) Vol. 10; no. 21; pp. 3756 - 3774
• Main Authors: Moriggi, Manuela, Vasso, Michele, Fania, Chiara, Capitanio, Daniele, Bonifacio, Gaetano, Salanova, Michele, Blottner, Dieter, Rittweger, Jörn, Felsenberg, Dieter, Cerretelli, Paolo, Gelfi, Cecilia
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley‐VCH Verlag 01.11.2010; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley-VCH
• Subjects: 2-D DIGE; Adult; Analytical, structural and metabolic biochemistry; Bed rest; Bed Rest - adverse effects; Biological and medical sciences; Biomedicine; Electrophoresis, Gel, Two-Dimensional; Exercise countermeasures; Exercise Therapy - methods; Fundamental and applied biological sciences. Psychology; Humans; Immunoblotting; Male; Mass Spectrometry; Miscellaneous; Muscle; Muscle, Skeletal - metabolism; Myosin Heavy Chains - chemistry; Myosin Heavy Chains - metabolism; Myosin Light Chains - chemistry; Myosin Light Chains - metabolism; Protein Isoforms - chemistry; Protein Isoforms - metabolism; Proteins; Proteomics - methods; Quadriceps Muscle - metabolism; Signal Transduction; Tandem Mass Spectrometry; Vibration - therapeutic use; Human; Biomedicine; 2-D DIGE; Proteomics; Muscle; Exercise countermeasures; Bed rest; Mass spectrometry; Protein

The present investigation, the first in the field, was aimed at analyzing differentially, on individual samples, the effects of 55 days of horizontal bed rest, a model for microgravity, on myosin heavy and myosin light chain isoforms distribution (by SDS) and on the proteome (by 2‐D DIGE and MS) in...