Effects of Fixation and Storage of Human Tissue Samples on Nucleic Acid Preservation
Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointes...
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| Published in | Journal of pathology and translational medicine Vol. 48; no. 1; pp. 36 - 42 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Korea (South)
Korean Society of Pathologists, Korean Society for Cytopathology
01.02.2014
The Korean Society of Pathologists and The Korean Society for Cytopathology 대한병리학회 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1738-1843 2383-7837 2092-8920 2383-7845 |
| DOI | 10.4132/KoreanJPathol.2014.48.1.36 |
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| Abstract | Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.
To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed.
All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks.
Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis. |
|---|---|
| AbstractList | Background: Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. Methods: To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed. Results: All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks. Conclusions: Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis. [PUBLICATION ABSTRACT] Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed. All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks. Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis. Background: Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. Methods: To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed. Results: All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, andamplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks. Conclusions: Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis. KCI Citation Count: 1 Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.BACKGROUNDBecause of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.To evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed.METHODSTo evaluate the effects of fixative, duration of fixation, decalcification, and storage periods on nucleic acid integrity, DNA and RNA were extracted from gastrointestinal cancer tissue. The yield and purity were analyzed, and polymerase chain reaction (PCR) for glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 60 bp), β-actin (148 bp), and human growth hormone (hGH; 434 bp) and real-time reverse transcription-PCR for β-actin (97 bp) were performed.All formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks.RESULTSAll formalin-fixed paraffin-embedded (FFPE) and methacarn-fixed paraffin-embedded (MFPE) samples tested positive for GAPDH and β-actin by PCR. hGH was successfully detected in all MFPE samples, but in only 46.7% of the FFPE samples. Prolonged formalin fixation resulted in fewer GAPDH and β-actin PCR products, and amplification of hGH was not successful. The PCR and reverse transcription-PCR results were significantly affected by the duration of decalcification. The yield, purity, and integrity of mRNA progressively decreased with increased storage periods of paraffin blocks.Fixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis.CONCLUSIONSFixation and storage should therefore be standardized in order to improve the quality of molecular pathologic diagnosis. |
| Author | Seo, An Na Nam, Soo Kyung Han, Nayoung Im, Joon Nam, Kyung Han Lee, Hye Seung Kwak, Yoonjin |
| AuthorAffiliation | 1 Department of Pathology, Seoul National University College of Medicine, Seoul, Korea Department of Pathology, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Korea |
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| Cites_doi | 10.1097/PDM.0b013e3181b520f8 10.1038/labinvest.3700459 10.1371/journal.pmed.1001453 10.2353/jmoldx.2006.050105 10.1002/(SICI)1097-0142(19971015)80:8+<1588::AID-CNCR9>3.0.CO;2-G 10.1200/JCO.2009.24.6116 10.1177/002215540205000113 10.1016/j.jmoldx.2011.02.004 10.1053/j.gastro.2009.12.064 10.1016/S0002-9440(10)64472-0 10.1097/00019606-199409000-00003 10.1007/s10689-012-9536-4 10.1097/00019606-199710000-00005 10.1016/S0027-5107(97)00314-X 10.1038/labinvest.3780023 10.1080/00313020802436410 10.1056/NEJMoa0804385 10.1200/JCO.2010.32.3550 |
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| Keywords | Fixation procedures Nucleic acid preservation Storage time |
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| References | Bardelli (10.4132/KoreanJPathol.2014.48.1.36_ref5) 2010; 28 Shao (10.4132/KoreanJPathol.2014.48.1.36_ref17) 2006; 86 Oh (10.4132/KoreanJPathol.2014.48.1.36_ref3) 2012; 11 Karapetis (10.4132/KoreanJPathol.2014.48.1.36_ref1) 2008; 359 Coleman (10.4132/KoreanJPathol.2014.48.1.36_ref16) 1997; 80 Boland (10.4132/KoreanJPathol.2014.48.1.36_ref4) 2010; 138 Guerrero (10.4132/KoreanJPathol.2014.48.1.36_ref13) 1997; 6 Foss (10.4132/KoreanJPathol.2014.48.1.36_ref11) 1994; 3 Botti (10.4132/KoreanJPathol.2014.48.1.36_ref6) 2008; 100 Dotti (10.4132/KoreanJPathol.2014.48.1.36_ref10) 2010; 19 Douglas (10.4132/KoreanJPathol.2014.48.1.36_ref15) 1998; 401 Talaulikar (10.4132/KoreanJPathol.2014.48.1.36_ref19) 2008; 40 Delfour (10.4132/KoreanJPathol.2014.48.1.36_ref8) 2006; 8 Lee (10.4132/KoreanJPathol.2014.48.1.36_ref14) 2011; 13 Shibutani (10.4132/KoreanJPathol.2014.48.1.36_ref9) 2000; 80 Marisa (10.4132/KoreanJPathol.2014.48.1.36_ref2) 2013; 10 Srinivasan (10.4132/KoreanJPathol.2014.48.1.36_ref7) 2002; 161 Jatoi (10.4132/KoreanJPathol.2014.48.1.36_ref18) 2011; 29 Brown (10.4132/KoreanJPathol.2014.48.1.36_ref12) 2002; 50 21704272 - J Mol Diagn. 2011 Jul;13(4):390-4 9362426 - Cancer. 1997 Oct 15;80(8 Suppl):1588-94 21555693 - J Clin Oncol. 2011 Jun 10;29(17):2301-4 23700391 - PLoS Med. 2013;10(5):e1001453 12466110 - Am J Pathol. 2002 Dec;161(6):1961-71 20420947 - Gastroenterology. 2010 Jun;138(6):2073-2087.e3 10701689 - Lab Invest. 2000 Feb;80(2):199-208 18792523 - Pathologica. 2008 Apr;100(2):76-85 20100961 - J Clin Oncol. 2010 Mar 1;28(7):1254-61 16940962 - Lab Invest. 2006 Oct;86(10):1089-95 9458386 - Diagn Mol Pathol. 1997 Oct;6(5):277-81 20502189 - Diagn Mol Pathol. 2010 Jun;19(2):112-22 7981889 - Diagn Mol Pathol. 1994 Sep;3(3):148-55 18985524 - Pathology. 2008 Dec;40(7):702-6 18946061 - N Engl J Med. 2008 Oct 23;359(17):1757-65 16645201 - J Mol Diagn. 2006 May;8(2):157-69 22669410 - Fam Cancer. 2012 Sep;11(3):459-66 11748301 - J Histochem Cytochem. 2002 Jan;50(1):113-5 9639679 - Mutat Res. 1998 Jun 5;401(1-2):77-88 |
| References_xml | – volume: 19 start-page: 112 year: 2010 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref10 publication-title: Diagn Mol Pathol doi: 10.1097/PDM.0b013e3181b520f8 – volume: 86 start-page: 1089 year: 2006 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref17 publication-title: Lab Invest doi: 10.1038/labinvest.3700459 – volume: 10 start-page: e1001453 year: 2013 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref2 publication-title: PLoS Med doi: 10.1371/journal.pmed.1001453 – volume: 8 start-page: 157 year: 2006 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref8 publication-title: J Mol Diagn doi: 10.2353/jmoldx.2006.050105 – volume: 80 start-page: 1588 issue: 8 Suppl year: 1997 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref16 publication-title: Cancer doi: 10.1002/(SICI)1097-0142(19971015)80:8+<1588::AID-CNCR9>3.0.CO;2-G – volume: 28 start-page: 1254 year: 2010 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref5 publication-title: J Clin Oncol doi: 10.1200/JCO.2009.24.6116 – volume: 50 start-page: 113 year: 2002 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref12 publication-title: J Histochem Cytochem doi: 10.1177/002215540205000113 – volume: 13 start-page: 390 year: 2011 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref14 publication-title: J Mol Diagn doi: 10.1016/j.jmoldx.2011.02.004 – volume: 138 start-page: 2073 year: 2010 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref4 publication-title: Gastroenterology doi: 10.1053/j.gastro.2009.12.064 – volume: 161 start-page: 1961 year: 2002 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref7 publication-title: Am J Pathol doi: 10.1016/S0002-9440(10)64472-0 – volume: 3 start-page: 148 year: 1994 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref11 publication-title: Diagn Mol Pathol doi: 10.1097/00019606-199409000-00003 – volume: 11 start-page: 459 year: 2012 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref3 publication-title: Fam Cancer doi: 10.1007/s10689-012-9536-4 – volume: 6 start-page: 277 year: 1997 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref13 publication-title: Diagn Mol Pathol doi: 10.1097/00019606-199710000-00005 – volume: 401 start-page: 77 year: 1998 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref15 publication-title: Mutat Res doi: 10.1016/S0027-5107(97)00314-X – volume: 100 start-page: 76 year: 2008 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref6 publication-title: Pathologica – volume: 80 start-page: 199 year: 2000 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref9 publication-title: Lab Invest doi: 10.1038/labinvest.3780023 – volume: 40 start-page: 702 year: 2008 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref19 publication-title: Pathology doi: 10.1080/00313020802436410 – volume: 359 start-page: 1757 year: 2008 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref1 publication-title: N Engl J Med doi: 10.1056/NEJMoa0804385 – volume: 29 start-page: 2301 year: 2011 ident: 10.4132/KoreanJPathol.2014.48.1.36_ref18 publication-title: J Clin Oncol doi: 10.1200/JCO.2010.32.3550 – reference: 9362426 - Cancer. 1997 Oct 15;80(8 Suppl):1588-94 – reference: 21704272 - J Mol Diagn. 2011 Jul;13(4):390-4 – reference: 23700391 - PLoS Med. 2013;10(5):e1001453 – reference: 12466110 - Am J Pathol. 2002 Dec;161(6):1961-71 – reference: 7981889 - Diagn Mol Pathol. 1994 Sep;3(3):148-55 – reference: 10701689 - Lab Invest. 2000 Feb;80(2):199-208 – reference: 18985524 - Pathology. 2008 Dec;40(7):702-6 – reference: 16645201 - J Mol Diagn. 2006 May;8(2):157-69 – reference: 22669410 - Fam Cancer. 2012 Sep;11(3):459-66 – reference: 18792523 - Pathologica. 2008 Apr;100(2):76-85 – reference: 20502189 - Diagn Mol Pathol. 2010 Jun;19(2):112-22 – reference: 11748301 - J Histochem Cytochem. 2002 Jan;50(1):113-5 – reference: 9639679 - Mutat Res. 1998 Jun 5;401(1-2):77-88 – reference: 18946061 - N Engl J Med. 2008 Oct 23;359(17):1757-65 – reference: 20420947 - Gastroenterology. 2010 Jun;138(6):2073-2087.e3 – reference: 21555693 - J Clin Oncol. 2011 Jun 10;29(17):2301-4 – reference: 20100961 - J Clin Oncol. 2010 Mar 1;28(7):1254-61 – reference: 16940962 - Lab Invest. 2006 Oct;86(10):1089-95 – reference: 9458386 - Diagn Mol Pathol. 1997 Oct;6(5):277-81 |
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| Snippet | Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.
To evaluate the effects... Background: Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice. Methods: To... Because of recent advances in the molecular diagnosis of cancer patients, tissue quality has become more important in daily practice.BACKGROUNDBecause of... |
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| StartPage | 36 |
| SubjectTerms | Chemicals Colorectal cancer Deoxyribonucleic acid DNA Genetic testing Growth hormones Methods Original Polymerase chain reaction Preservation Studies 병리학 |
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| Title | Effects of Fixation and Storage of Human Tissue Samples on Nucleic Acid Preservation |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/24627693 https://www.proquest.com/docview/1508552834 https://www.proquest.com/docview/1507791365 https://pubmed.ncbi.nlm.nih.gov/PMC3950233 https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART001852712 |
| Volume | 48 |
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| ispartofPNX | Journal of Pathology and Translational Medicine, 2014, 48(1), , pp.36-42 |
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