A Genetic Code Expansion‐Derived Molecular Beacon for the Detection of Intracellular Amyloid‐β Peptide Generation

• Published in: Angewandte Chemie International Edition Vol. 60; no. 8; pp. 3934 - 3939
• Main Authors: Sappakhaw, Khomkrit, Jantarug, Krittapas, Slavoff, Sarah A., Israsena, Nipan, Uttamapinant, Chayasith
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 19.02.2021; John Wiley and Sons Inc
• Edition: International ed. in English
• Subjects: Alzheimer's disease; Amino Acyl-tRNA Synthetases - genetics; Amino Acyl-tRNA Synthetases - metabolism; Amyloid beta-Peptides - chemistry; Amyloid beta-Peptides - metabolism; Amyloid beta-Protein Precursor - genetics; Amyloid beta-Protein Precursor - metabolism; Amyloid precursor protein; amyloids; Archaeal Proteins - genetics; Archaeal Proteins - metabolism; Communication; Communications; Genetic Code; genetic code expansion; HEK293 Cells; Humans; Labeling; Lysine - analogs & derivatives; Lysine - chemistry; Lysine - metabolism; Methanosarcina - enzymology; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Neurodegenerative diseases; Pathogenesis; Peptides; Polypeptides; Precursors; Protein Processing, Post-Translational; Proteins; proteoforms; Proteolysis; proteolytic processing; β-Amyloid; amyloids; proteoforms; genetic code expansion; proteolytic processing

Polypeptides generated from proteolytic processing of protein precursors, or proteolytic proteoforms, play an important role in diverse biological functions and diseases. However, their often‐small size and intricate post‐translational biogenesis preclude the use of simple genetic tagging in their c...