Intracellular Crowding by Bio‐Orthogonal Hydrogel Formation Induces Reversible Molecular Stasis

• Published in: Advanced materials (Weinheim) Vol. 34; no. 31; pp. e2202882 - n/a
• Main Authors: Macdougall, Laura J., Hoffman, Timothy E., Kirkpatrick, Bruce E., Fairbanks, Benjamin D., Bowman, Christopher N., Spencer, Sabrina L., Anseth, Kristi S.
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.08.2022
• Subjects: Alkynes; Alkynes - chemistry; Animals; Azides - chemistry; biostasis; Cell death; Cellular structure; click chemistry; Crosslinking; Cycloaddition; Hydrogels; Hydrogels - chemistry; intracellular crosslinking; Mammals; Materials science; poly(ethylene glycol); Polyethylene glycol; Polyethylene Glycols - chemistry; Polymers; Polymers - chemistry; Polysaccharides; Protein synthesis; Proteins; Vitrification; intracellular crosslinking; click chemistry; biostasis; hydrogels; poly(ethylene glycol)

To survive extreme conditions, certain animals enter a reversible protective stasis through vitrification of the cytosol by polymeric molecules such as proteins and polysaccharides. In this work, synthetic gelation of the cytosol in living cells is used to induce reversible molecular stasis. Through...