Synthesis of deuterium-labeled atorvastatin and its metabolites for use as internal standards in a LC/MS/MS method developed for quantitation of the drug and its metabolites in human serum

• Published in: Journal of labelled compounds & radiopharmaceuticals Vol. 43; no. 3; pp. 261 - 270
• Main Authors: Chen, Bang-Chi, Sundeen, Joseph E., Guo, Peng, Bednarz, Mark S., Hangeland, Jon J., Ahmed, Syed Z., Jemal, Mohammed
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 15.03.2000; Wiley
• Subjects: Analysis; atorvastatin; atorvastatin lactone; Biochemical Research Methods; Biochemistry & Molecular Biology; Biological and medical sciences; Chemistry; Chemistry, Analytical; Chemistry, Medicinal; deuterium; Exact sciences and technology; General pharmacology; Heterocyclic compounds; Heterocyclic compounds with only one n hetero atom and condensed derivatives; hydroxy metabolites; Life Sciences & Biomedicine; Medical sciences; Organic chemistry; Pharmacology & Pharmacy; Pharmacology. Drug treatments; Physical Sciences; Preparations and properties; Science & Technology; atorvastatin lactone; hydroxy metabolites; POTENT; PHARMACOKINETICS; deuterium; HEALTHY-SUBJECTS; HMG-COA REDUCTASE; INHIBITOR; atorvastatin; Deuterium; Biological fluid; Diol; Isotope labelling; Nitrogen heterocycle; Metabolite; Internal standard; Pyran derivatives; Lactone; Liquid chromatography; Hydrogen Isotopes; Blood; Pyrrole derivatives; Hydroxyacid; Mass spectrometry MS/MS; Simultaneous measurement; Atorvastatin; Phenols; Carboxamide; Chemical synthesis; Blood serum; Quantitative analysis
• ISSN: 0362-4803; 1099-1344
• DOI: 10.1002/(SICI)1099-1344(20000315)43:3<261::AID-JLCR312>3.0.CO;2-T

D5‐labeled isotopomers of atorvastatin, atorvastatin lactone and its hydroxy metabolites were synthesized as internal standards for use in a LC/MS/MS method developed for the simultaneous quantitative determination of atorvastatin and its hydroxy metabolites in human serum. d5‐Atorvastatin and d5‐atorvastatin lactone were prepared from d5‐aniline whereas their corresponding hydroxy metabolites were synthesized using d5‐benzaldehyde. Copyright © 2000 John Wiley & Sons, Ltd.