Identification of differentially expressed genes in the testis of Sprague-Dawley rats treated with di( n -butyl) phthalate

• Published in: Toxicology (Amsterdam) Vol. 234; no. 1; pp. 103 - 112
• Main Authors: Ryu, Ju Young, Lee, Byung Mu, Kacew, Sam, Kim, Hyung Sik
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 05.05.2007; Amsterdam Elsevier Science
• Subjects: Administration, Oral; Age Factors; Animals; Biological and medical sciences; Blotting, Western; Di( n-butyl) phthalate; Dibutyl Phthalate - administration & dosage; Dibutyl Phthalate - toxicity; Differentially expressed genes; Dose-Response Relationship, Drug; Emergency; Estrogen Receptor beta - genetics; Estrogen Receptor beta - metabolism; Gene Expression Profiling - methods; Gene Expression Regulation - drug effects; Genes, erbA - drug effects; Genes, erbA - genetics; Male; Medical sciences; Organ Size - drug effects; Plasticizers - administration & dosage; Plasticizers - toxicity; PPAR alpha - genetics; PPAR alpha - metabolism; Rats; Rats, Sprague-Dawley; Receptors, Androgen - genetics; Receptors, Androgen - metabolism; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Spermatogenesis; Spermatogenesis - drug effects; Spermatogenesis - genetics; Steroidogenesis; Testis; Testis - drug effects; Testis - metabolism; Testis - pathology; Toxicology; Testis; Di( n-butyl) phthalate; Differentially expressed genes; Steroidogenesis; Spermatogenesis; Rat; Rodentia; Phthalate; Di(n-butyl) phthalate; Gene expression; Testicle; Male genital system; Vertebrata; Mammalia; Treatment; Animal

Abstract The aim of this study was to identify the di( n -butyl) phthalate (DBP)-induced differentially expressed genes (DEGs) using a novel annealing control primer system in the testes of Sprague-Dawley male rats. Animals (4 weeks of age) were administered orally either corn oil only (vehicle cont...