Plasmon-enhanced fluorescence spectroscopy
Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fl...
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| Published in | Chemical Society reviews Vol. 46; no. 13; pp. 3962 - 3979 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
England
03.07.2017
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| Subjects | |
| Online Access | Get full text |
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| Abstract | Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties.
Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. |
|---|---|
| AbstractList | Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties. Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties. Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties.Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level. Plasmon-enhanced fluorescence (PEF) not only offers enhanced emissions and decreased lifetimes, but also allows an expansion of the field of fluorescence by incorporating weak quantum emitters, avoiding photobleaching and providing the opportunity of imaging with resolutions significantly better than the diffraction limit. It also opens the window to a new class of photostable probes by combining metal nanostructures and quantum emitters. In particular, the shell-isolated nanostructure-enhanced fluorescence, an innovative new mode for plasmon-enhanced surface analysis, is included. These new developments are based on the coupling of the fluorophores in their excited states with localized surface plasmons in nanoparticles, where local field enhancement leads to improved brightness of molecular emission and higher detection sensitivity. Here, we review the recent progress in PEF with an emphasis on the mechanism of plasmon enhancement, substrate preparation, and some advanced applications, including an outlook on PEF with high time- and spatially resolved properties. |
| Author | Li, Jian-Feng Li, Chao-Yu Aroca, Ricardo F |
| AuthorAffiliation | Department of Chemistry University of Chile State Key Laboratory of Physical Chemistry of Solid Surfaces iChEM MOE Key Laboratory of Spectrochemical Analysis and Instrumentation Research Institute for Biomimetics and Soft Matter Department of Chemistry and Biochemistry Xiamen University College of Chemistry and Chemical Engineering University of Windsor Faculty of Science Department of Physics |
| AuthorAffiliation_xml | – name: Research Institute for Biomimetics and Soft Matter – name: MOE Key Laboratory of Spectrochemical Analysis and Instrumentation – name: University of Windsor – name: Department of Chemistry – name: iChEM – name: College of Chemistry and Chemical Engineering – name: Department of Physics – name: State Key Laboratory of Physical Chemistry of Solid Surfaces – name: University of Chile – name: Xiamen University – name: Faculty of Science – name: Department of Chemistry and Biochemistry |
| Author_xml | – sequence: 1 givenname: Jian-Feng surname: Li fullname: Li, Jian-Feng – sequence: 2 givenname: Chao-Yu surname: Li fullname: Li, Chao-Yu – sequence: 3 givenname: Ricardo F surname: Aroca fullname: Aroca, Ricardo F |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28639669$$D View this record in MEDLINE/PubMed |
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| Notes | Jian-Feng Li is a Professor of Chemistry at Xiamen University. He received a BSc in chemistry from Zhejiang University, and a PhD in chemistry from Xiamen University. Professor Li is the principal inventor of shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS). His research interests include surface-enhanced Raman spectroscopy, surface-enhanced fluorescence, core-shell nanostructures, surface plasmon resonance, electrochemistry and surface catalysis. Chao-Yu Li is now pursuing his PhD degree under the supervision of Prof. Zhong-Qun Tian and Prof. Jian-Feng Li at Xiamen University. His research is focused on spectroelectrochemistry, synthesis of plasmonic nanostructures, and plasmon-enhanced single-molecule spectroscopy. Dr Ricardo Aroca is Professor Emeritus in the Department of Chemistry and Biochemistry at the University of Windsor, and Adjunct Professor in the Department of Chemistry, Faculty of Science, University of Chile. In 2003 he was honoured with the Gerhard Herzberg Award in recognition of outstanding achievement in the science of spectroscopy. In 2005 he was elected Fellow of the Chemical Institute of Canada. Since 2009, he has also been a Member Correspondent of the Chilean Academy of Sciences. His research is on plasmon enhanced spectroscopy. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 ObjectType-Review-3 content type line 23 |
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| Snippet | Fluorescence spectroscopy with strong emitters is a remarkable tool with ultra-high sensitivity for detection and imaging down to the single-molecule level.... |
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| SubjectTerms | Coupling (molecular) detection limit emissions Emitters Fluorescence fluorescence emission spectroscopy fluorescent dyes image analysis Imaging nanoparticles Nanostructure photobleaching Plasmons Sensitivity Spectroscopy |
| Title | Plasmon-enhanced fluorescence spectroscopy |
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