Preparation and characterization of PEGyated Concanavalin A for affinity chromatography with improved stability

In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylat...

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Bibliographic Details
Published inJournal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 879; no. 20; pp. 1732 - 1740
Main Authors Wen, Zhenzhen, Niemeyer, Bernd
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.06.2011
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