Preparation and characterization of PEGyated Concanavalin A for affinity chromatography with improved stability

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 879; no. 20; pp. 1732 - 1740
• Main Authors: Wen, Zhenzhen, Niemeyer, Bernd
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2011
• Subjects: Adsorbents; Adsorption; Affinity adsorption; affinity chromatography; Binding; binding capacity; biocatalysis; bioprocessing; carbohydrate binding; chemical bonding; Chloroform - chemistry; Chromatography, Affinity - methods; Colorimetry; Concanavalin A; Concanavalin A - chemistry; Concanavalin A - metabolism; drug therapy; ethylene glycol; Glucose oxidase; Glucose Oxidase - chemistry; Immobilized Proteins - chemistry; Immobilized Proteins - metabolism; Linear Models; Methanol - chemistry; Molecular weight; MPEG encoders; Ninhydrin - chemistry; PEGylation; polyethylene glycol; Polyethylene Glycols - analysis; Polyethylene Glycols - chemistry; propionic acid; Protein Stability; Reproducibility of Results; Separation; solvents; sorption isotherms; Stability; Temperature; Glucose oxidase; C; PVPAA; Con A; HRP; Protein stability; DP; Concanavalin A; M; Affinity adsorption; TC; Ads; mPEG-SPA; PEGylation; GOD

In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylat...