Calcineurin Regulates Coelomocyte Endocytosis via DYN-1 and CUP-4 in Caenorhabditis elegans

• Published in: Molecules and cells Vol. 30; no. 3; pp. 255 - 262
• Main Authors: Song, H.O., Hanyang University, Seoul, Republic of Korea, Lee, J.S., Hanyang University, Seoul, Republic of Korea, Ji, Y.J., Hanyang University, Seoul, Republic of Korea, Dwivedi, Meenakshi, Hanyang University, Seoul, Republic of Korea, Cho, J.H., Chosun University, Gwangju, Republic of Korea, Park, B.J., Hallym University, Chuncheon, Republic of Korea, Ahnn, J.H., Hanyang University, Seoul, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Springer Korean Society for Molecular and Cellular Biology 01.09.2010; 한국분자세포생물학회
• Subjects: Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Biotechnology; CAENORHABDITIS ELEGANS; Caenorhabditis elegans - physiology; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Calcineurin - genetics; Calcineurin - metabolism; Calcium - metabolism; Cell Biology; Cells, Cultured; Dynamins - metabolism; ENDOCITOSIS; ENDOCYTOSE; ENDOCYTOSIS; Endocytosis - genetics; Life Sciences; Mutation - genetics; phosphatase; Signal Transduction; 생물학; endocytosis; phosphatase; signaling; Ca
• ISSN: 1016-8478; 0219-1032
• DOI: 10.1007/s10059-010-0116-x

C. elegans coelomocytes are macrophage-like scavenger cells that provide an excellent in vivo system for the study of clathrin-mediated endocytosis. Using this in vivo system, several genes involved in coelomocyte endocytosis have been identified previously. However, the detailed mechanism of endocytic pathway is still unknown. Here, we report a new function of calcineurin, an evolutionarily conserved Ca²+/calmodulin-dependent Ser/Thr protein phosphatase, in coelomocyte endocytosis. We found that calcineurin mutants show defective coelomocyte endocytosis. Genetic analysis suggests that calcineurin and a GTPase, dynamin (DYN-1), may function upstream of an orphan receptor, CUP-4, to regulate endocytosis. Therefore, we propose a model in which calcineurin may regulate coelomocyte endocytosis via DYN-1 and CUP-4 in C. elegans.