Efficacious bioconversion of waste walnut shells to xylotetrose and xylopentose by free xylanase (Xy) and MOF immobilized xylanase (Xy-Cu-BTC)

• Published in: Bioresource technology Vol. 357; p. 127374
• Main Authors: Kaushal, Jyoti, Arya, Shailendra Kumar, Khatri, Madhu, Singh, Gursharan, Izyan Wan Azelee, Nur, Rajagopal, Rajinikanth, Woong Chang, Soon, Ravindran, Balasubramani, Kumar Awasthi, Mukesh
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2022
• Subjects: biotransformation; coordination polymers; copper; cost effectiveness; Hemicellulosic; Immobilization; Metal organic framework; polymerization; technology; walnuts; xylan; Xylanase; xylanases; xylooligosaccharides; Xylooligosaccharides (XOS); Xylopentose; xylose; Xylotetrose; Metal organic framework; Xylanase; Hemicellulosic; Xylotetrose; Immobilization; Xylooligosaccharides (XOS); Xylopentose; immobilization; metal organic framework; hemicellulosic; xylanase
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2022.127374

[Display omitted] •Xylanase (Xy) extracted from Bacillus pumilus bacterial strain was partially purified.•Production of Cu-BTC MOF was done at room temperature.•Xy was immobilized onto Cu-BTC MOF to form blue crystals of Xy-Cu-BTC.•Xylan was extracted from waste walnut shell.•Bioconversion of xylan to xylooligosaccharides by free and immobilized enzyme system. This study uses a cost effective and efficient method for production of higher DP (degree of polymerization) Xylooligosaccharides (XOS) from xylan extracted from the waste walnut shells. Copper based metal organic framework (Cu-BTC MOF) was prepared for immobilization of free xylanase (Xy) enzyme by green synthesis method. Both free and immobilized xylanase (Xy-Cu-BTC) were able to cause the bioconversion of xylan (87.4% yield) into XOS. Predominant production of xylotetrose (X4) and xylopentose (X5) was observed for both the methods. Percentage XOS conversion for free enzyme (Xy) was found to be 4.1% X4 and 60.57% X5 whereas these values increased in case of immobilized system where 11.8% X4 and 64.2% X5 were produced. Xylose production was minute in case of immobilized xylanase 0.88% which makes it a better method for XOS production free from xylose interference. Xy-Cu-BTC MOF can hence be used as an attractive alternative for pure XOS production.