Analysis of protein denaturation, aggregation and post-translational modification by agarose native gel electrophoresis

• Published in: International journal of biological macromolecules Vol. 172; pp. 589 - 596
• Main Authors: Sakuma, Chiaki, Tomioka, Yui, Li, Cynthia, Shibata, Takashi, Nakagawa, Masataka, Kurosawa, Yasunori, Arakawa, Tsutomu, Akuta, Teruo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2021
• Subjects: Agarose; Animals; antibodies; Antibody; bovine serum albumin; BSA; Cattle; Dependovirus - genetics; Dependovirus - metabolism; disulfides; Electrophoresis, Polyacrylamide Gel - methods; gel electrophoresis; gels; Glycated Hemoglobin A - genetics; Glycated Hemoglobin A - metabolism; glycohemoglobin; heat treatment; Hemoglobin; Humans; Hydrogen Peroxide - pharmacology; Native gel; Phosphorylation; post-translational modification; Protein Aggregates; Protein Denaturation; Protein Processing, Post-Translational; Proteolysis; Serum Albumin, Bovine - genetics; Serum Albumin, Bovine - metabolism; sodium dodecyl sulfate; Sodium Dodecyl Sulfate - chemistry; Transferrin; Transferrin - genetics; Transferrin - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; viruses; ZAP-70 Protein-Tyrosine Kinase - genetics; ZAP-70 Protein-Tyrosine Kinase - metabolism; Hemoglobin; BSA; Transferrin; Native gel; Antibody; Agarose

Agarose native gel electrophoresis has been developed to separate proteins and protein complexes in the native state. Here, we applied this technology to analyze proteins that undergo degradation, post-translational modification or chemical/physical changes. Antibodies showed aggregation/association...