Cytomegalovirus quantification in plasma by an automated real-time PCR assay

• Published in: Journal of clinical virology Vol. 38; no. 4; pp. 298 - 303
• Main Authors: Yerly, Sabine, Perrin, Luc, Van Delden, Christian, Schaffer, Sven, Thamm, Sven, Wunderli, Werner, Kaiser, Laurent
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2007; Elsevier Science
• Subjects: Adult; Allergy and Immunology; Analysis of Variance; Biological and medical sciences; Cytomegalovirus; Cytomegalovirus - genetics; DNA, Viral - blood; Female; Fundamental and applied biological sciences. Psychology; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Miscellaneous; Plasma - virology; Plasma CMV DNA; Polymerase Chain Reaction - methods; Real-time PCR; Reproducibility of Results; Sensitivity and Specificity; Viral diseases; Viral Load - methods; Virology; Real-time PCR; Cytomegalovirus; Plasma CMV DNA; Microbiology; Herpesviridae; Real time; Betaherpesvirinae; Virology; Infection; Virus; Polymerase chain reaction; Viral disease; Quantitative analysis

Abstract Background Sensitive quantitation of cytomegalovirus (CMV) DNA in blood is helpful for the diagnosis of CMV infection or reactivation and the monitoring of transplanted patients. Objectives We compared a new PCR assay coupled with an automated extraction system (CMV real-time PCR, Abbott Mo...