Development of plant cytosine base editors with the Cas12a system

• Published in: The Crop journal Vol. 11; no. 5; pp. 1451 - 1457
• Main Authors: Wang, Huanhuan, Liang, Jing, Chen, Like, Deng, Bufang, Gu, Dongfang, Liu, Xiaoshuang, Jin, Shan, Xu, Rongfang, Qin, Ruiying, Zhu, Yitong, Zhao, Liangxia, Kou, Dourong, Chen, Yanjun, Jiang, Yingli, Li, Juan, Wei, Pengcheng
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2023; KeAi Communications Co., Ltd
• Subjects: ABE; adenine; Base editing; Cas12a; CBE; cytosine; DNA-binding domains; genetically modified organisms; genomics; humans; Rice; Cas12a; ABE; Base editing; CBE; Rice

Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs). The main elements of Cas12a-CBEs were engineered and their efficiency was evaluated in stably transformed rice cells....