Intracellular trafficking of hybrid gene delivery vectors

• Published in: Journal of controlled release Vol. 207; pp. 120 - 130
• Main Authors: Keswani, Rahul K., Lazebnik, Mihael, Pack, Daniel W.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 10.06.2015
• Subjects: Blood Proteins - metabolism; Caveolae - metabolism; Chitosan; Chitosan - chemistry; Chitosan - metabolism; Cholesterol; Cholesterol - chemistry; Cholesterol - metabolism; Clathrin-Coated Vesicles - metabolism; DOPE; DOTAP; Endocytosis - drug effects; Fatty Acids, Monounsaturated - chemistry; Fatty Acids, Monounsaturated - metabolism; Gene therapy; Genetic Vectors; HEK293 Cells; HeLa Cells; Humans; Hybrid vectors; Kinetics; Liposomes; Microscopy, Confocal; Moloney murine leukemia virus - genetics; Moloney murine leukemia virus - metabolism; Phosphatidylethanolamines - chemistry; Phosphatidylethanolamines - metabolism; Pinocytosis; Quaternary Ammonium Compounds - chemistry; Quaternary Ammonium Compounds - metabolism; Retrovirus; Temperature; Transfection - methods; Virion - genetics; Virion - metabolism; DOPE; Retrovirus; Hybrid vectors; Chitosan; Liposomes; DOTAP; Gene therapy; Cholesterol

Viral and non-viral gene delivery vectors are in development for human gene therapy, but both exhibit disadvantages such as inadequate efficiency, lack of cell-specific targeting or safety concerns. We have recently reported the design of hybrid delivery vectors combining retrovirus-like particles w...