Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

• Published in: Journal of veterinary diagnostic investigation Vol. 32; no. 6; p. 815
• Main Authors: Toohey-Kurth, Kathy L, Mulrooney, Donna M, Hinkley, Susanne, Lea Killian, Mary, Pedersen, Janice C, Bounpheng, Mangkey A, Pogranichniy, Roman, Bolin, Steve, Maes, Roger, Tallmadge, Rebecca L, Goodman, Laura B, Crossley, Beate M
• Format: Journal Article
• Language: English
• Published: United States 01.11.2020
• Subjects: Animals; Laboratories - standards; Quality Control; Real-Time Polymerase Chain Reaction - veterinary; Sensitivity and Specificity; qPCR; real-time PCR; AAVLD; practice guidelines

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter expe...