Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter expe...

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Published inJournal of veterinary diagnostic investigation Vol. 32; no. 6; p. 815
Main Authors Toohey-Kurth, Kathy L, Mulrooney, Donna M, Hinkley, Susanne, Lea Killian, Mary, Pedersen, Janice C, Bounpheng, Mangkey A, Pogranichniy, Roman, Bolin, Steve, Maes, Roger, Tallmadge, Rebecca L, Goodman, Laura B, Crossley, Beate M
Format Journal Article
LanguageEnglish
Published United States 01.11.2020
Subjects
Animals
Laboratories - standards
Quality Control
Real-Time Polymerase Chain Reaction - veterinary
Sensitivity and Specificity
qPCR
real-time PCR
AAVLD
practice guidelines
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Summary:The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.
ISSN:1943-4936
DOI:10.1177/1040638720962076