Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories
The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter expe...
Saved in:
| Published in | Journal of veterinary diagnostic investigation Vol. 32; no. 6; p. 815 |
|---|---|
| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.11.2020
|
| Subjects | |
| Online Access | Get more information |
Cover
Loading…
| Abstract | The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature. |
|---|---|
| AbstractList | The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature. |
| Author | Hinkley, Susanne Bolin, Steve Lea Killian, Mary Mulrooney, Donna M Pedersen, Janice C Maes, Roger Tallmadge, Rebecca L Goodman, Laura B Bounpheng, Mangkey A Pogranichniy, Roman Toohey-Kurth, Kathy L Crossley, Beate M |
| Author_xml | – sequence: 1 givenname: Kathy L orcidid: 0000-0002-6212-3263 surname: Toohey-Kurth fullname: Toohey-Kurth, Kathy L organization: California Animal Health and Food Safety Laboratory, University of California-Davis, San Bernardino – sequence: 2 givenname: Donna M surname: Mulrooney fullname: Mulrooney, Donna M organization: Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, OR – sequence: 3 givenname: Susanne surname: Hinkley fullname: Hinkley, Susanne organization: Neogen Corporation, Lincoln, NE – sequence: 4 givenname: Mary surname: Lea Killian fullname: Lea Killian, Mary organization: National Veterinary Services Laboratories, Ames, IA – sequence: 5 givenname: Janice C surname: Pedersen fullname: Pedersen, Janice C organization: National Veterinary Services Laboratories, Ames, IA – sequence: 6 givenname: Mangkey A surname: Bounpheng fullname: Bounpheng, Mangkey A organization: Texas A&M University, College Station, TX – sequence: 7 givenname: Roman surname: Pogranichniy fullname: Pogranichniy, Roman organization: Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, KS – sequence: 8 givenname: Steve surname: Bolin fullname: Bolin, Steve organization: Veterinary Diagnostic Laboratory, Michigan State University, Lansing, MI – sequence: 9 givenname: Roger surname: Maes fullname: Maes, Roger organization: Veterinary Diagnostic Laboratory, Michigan State University, Lansing, MI – sequence: 10 givenname: Rebecca L orcidid: 0000-0002-7466-5449 surname: Tallmadge fullname: Tallmadge, Rebecca L organization: Population Medicine & Diagnostic Sciences, Cornell University, Ithaca, NY – sequence: 11 givenname: Laura B orcidid: 0000-0002-8327-3092 surname: Goodman fullname: Goodman, Laura B organization: Population Medicine & Diagnostic Sciences, Cornell University, Ithaca, NY – sequence: 12 givenname: Beate M orcidid: 0000-0003-2932-7229 surname: Crossley fullname: Crossley, Beate M organization: California Animal Health and Food Safety Laboratory, University of California-Davis, San Bernardino and Davis branches, CA |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32996402$$D View this record in MEDLINE/PubMed |
| BookMark | eNo1j8tKxDAUQIMozkP3riQ_UM3zpllqcVQYUER3wpCktxKZpiWpwvy9BXV1dodzVuQ4DQkJueDsinNjrjlTDGRtBLMgmIEjsuRWyUpZCQuyKuWTMS204adkIYW1oJhYkvdbLBMdswtTDFhoN2Q6Yp7RuxSQDh3N6PbVFHukz80LdaW4Q6Ex0W-cMMfk8oG20X2kocwKund-yG4acsRyRk46ty94_sc1edvcvTYP1fbp_rG52VZBgZmquVIG0Jbpugv1_OIQJDj0wWMtYd5pQXPrVQ0qBOShZh0y1kplvTAaxJpc_nrHL99juxtz7Oes3f-m-AFO31UN |
| CitedBy_id | crossref_primary_10_1177_1040638720960829 crossref_primary_10_3389_frmbi_2024_1446394 crossref_primary_10_1016_j_cvfa_2022_10_006 crossref_primary_10_3390_microorganisms13010054 crossref_primary_10_3390_pathogens11040404 crossref_primary_10_1111_vde_13299 crossref_primary_10_1177_10406387241230315 crossref_primary_10_1111_jvim_16958 |
| ContentType | Journal Article |
| DBID | CGR CUY CVF ECM EIF NPM |
| DOI | 10.1177/1040638720962076 |
| DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) |
| DatabaseTitleList | MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Veterinary Medicine |
| EISSN | 1943-4936 |
| ExternalDocumentID | 32996402 |
| Genre | Journal Article |
| GroupedDBID | --- -TM .2E .2J .2N 01A 0R~ 1~K 2WC 53G 54M 5GY 5RE 5VS AABMB AACKU AACMV AACTG AADUE AAEJI AAEWN AAGGD AAGLT AAGMC AAHBH AAJIQ AAJOX AAJPV AAKGS AANSI AAPEO AAPII AAQXH AAQXI AARDL AARIX AATAA AATBZ AAUAS AAYTG ABAWP ABCCA ABCJG ABDWY ABEIX ABFWQ ABHKI ABIDT ABJNI ABJZC ABKRH ABLUO ABPGX ABPNF ABQKF ABQXT ABRHV ABUJY ABVFX ABYTW ACARO ACDSZ ACDXX ACFEJ ACFMA ACGBL ACGFS ACGZU ACJER ACJTF ACLFY ACLHI ACLZU ACOFE ACOXC ACROE ACSIQ ACUAV ACUIR ACXKE ACXMB ADBBV ADDLC ADEBD ADEIA ADNON ADRRZ ADTBJ ADUKL ADVBO ADZZY AECGH AECVZ AEDTQ AEKYL AENEX AEPTA AEQLS AERKM AESZF AEUHG AEWDL AEWHI AEXNY AFEET AFKBI AFKRG AFMOU AFQAA AFUIA AGHKR AGKLV AGNHF AGPXR AGWFA AHDMH AHJOV AJEFB AJGYC AJMMQ AJUZI AJVBE AJXAJ ALKWR ALMA_UNASSIGNED_HOLDINGS AMCVQ ANDLU AOIJS ARTOV AUTPY AUVAJ AYAKG B8M BAWUL BBRGL BDDNI BKIIM BKSCU BPACV BSEHC BWJAD CBRKF CDWPY CFDXU CGR CORYS CQQTX CUY CVF DB0 DC- DC. DD- DD0 DE- DE. DF0 DN0 DO- DOPDO DV7 E3Z EBS ECGQY ECM EIF EJD EYRJQ F5P FHBDP GROUPED_SAGE_PREMIER_JOURNAL_COLLECTION H13 HYE HZ~ J8X K.F NPM O9- OK1 P.9 P.B P2P Q1R Q7L Q7U Q83 ROL S01 SASJQ SAUOL SCNPE SFC SHG SPQ SPV TR2 TVL W8F WOQ ZONMY ZPPRI ZRKOI ZSSAH ~KM |
| ID | FETCH-LOGICAL-c467t-9433c659058fc8177ae636aebcbe836962d6519b4864cce1c80fe00d349b27562 |
| IngestDate | Mon Jul 21 05:51:37 EDT 2025 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | qPCR real-time PCR AAVLD practice guidelines |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c467t-9433c659058fc8177ae636aebcbe836962d6519b4864cce1c80fe00d349b27562 |
| ORCID | 0000-0002-8327-3092 0000-0002-7466-5449 0000-0003-2932-7229 0000-0002-6212-3263 |
| OpenAccessLink | https://journals.sagepub.com/doi/pdf/10.1177/1040638720962076 |
| PMID | 32996402 |
| ParticipantIDs | pubmed_primary_32996402 |
| PublicationCentury | 2000 |
| PublicationDate | 2020-11-01 |
| PublicationDateYYYYMMDD | 2020-11-01 |
| PublicationDate_xml | – month: 11 year: 2020 text: 2020-11-01 day: 01 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Journal of veterinary diagnostic investigation |
| PublicationTitleAlternate | J Vet Diagn Invest |
| PublicationYear | 2020 |
| SSID | ssj0052571 |
| Score | 2.319207 |
| Snippet | The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and... |
| SourceID | pubmed |
| SourceType | Index Database |
| StartPage | 815 |
| SubjectTerms | Animals Laboratories - standards Quality Control Real-Time Polymerase Chain Reaction - veterinary Sensitivity and Specificity |
| Title | Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32996402 |
| Volume | 32 |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LS8QwEA6rgngR32_JwdtSzbZpmh51VRZfiKziQZA2SVHQVmQV1j_k33SStE1ZFR-XsjSk7Pb7NpmZfDOD0JbMKEt5rNukUgoOSkQ8HorMY6HwpYhUJFOdO3x6xnqX9Og6vG613huqpZdBui3evswr-Q-qcA9w1Vmyf0C2fijcgM-AL1wBYbj-CuM9WNLrPCdTWEGXIa4TAcAOBJPwwdP949vn3Ys2GMrJ0AhgX7UKxqbiSiu204VbS0YUz5Wy8LPV-vXEe1etwx3r94viTg2945dShKiFG8P2iYP4Aaz2Umy2X-R50ojMgodcBtONbMid_Z-opH1sYkR5mWo0bAYuwEvt1IELZRfbmAYejW0BlGo1dtHOkaWV27TPz0u-OXQGr1IbX5EPLplPbEuZBgOeHg0FAth8GTUp3j-MjhThrobG0Bi4I7q_qg4K2Q1f15PtuBPwndGvMoUmq-kjvouxYfozaLqEEe9aJs2ilsrn0NxVDSk-LZUW8-hGcwvX3MJAKtzgFi4yXHMLA7ew5Ra-z7GjCHYUwU1uLaDLw4N-t-eVnTg8ARvpwAOgAsHCmIQ8Exx-ZKJYwBKVilRx3RHSlwxcgZRyRoVQHcFJpgiRAY1T3V_AX0TjOTBqGWEhM0Z5rFu-SJpEJCERPFImMI3RmNAVtGRf0e2TLbdyW7281W9H1tCUI9g6msjg_602wFgcpJsGqg96J22D |
| linkProvider | National Library of Medicine |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Best+practices+for+performance+of+real-time+PCR+assays+in+veterinary+diagnostic+laboratories&rft.jtitle=Journal+of+veterinary+diagnostic+investigation&rft.au=Toohey-Kurth%2C+Kathy+L&rft.au=Mulrooney%2C+Donna+M&rft.au=Hinkley%2C+Susanne&rft.au=Lea+Killian%2C+Mary&rft.date=2020-11-01&rft.eissn=1943-4936&rft.volume=32&rft.issue=6&rft.spage=815&rft_id=info:doi/10.1177%2F1040638720962076&rft_id=info%3Apmid%2F32996402&rft_id=info%3Apmid%2F32996402&rft.externalDocID=32996402 |