Development of a duplex real-time RT-PCR assay for the detection and identification of two subgroups of human metapneumovirus in a single tube

• Published in: Journal of virological methods Vol. 322; p. 114812
• Main Authors: Sugimoto, Satoko, Kawase, Miyuki, Suwa, Reiko, Kakizaki, Masatoshi, Kume, Yohei, Chishiki, Mina, Ono, Takashi, Okabe, Hisao, Norito, Sakurako, Hosoya, Mitsuaki, Hashimoto, Koichi, Shirato, Kazuya
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2023
• Subjects: Child; cross reaction; detection limit; Duplex assay; genomics; Human metapneumovirus; Humans; Infant; Japan; Metapneumovirus - genetics; monitoring; Paramyxoviridae Infections - diagnosis; Real-time RT-PCR; Respiratory Tract Infections; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity; species; Subgroup; Real-time RT-PCR; HCoV; HBoV1; Subgroup; Cp; HRV; Flu; COVID-19; NIID; SARS-CoV-2; ADV; HPIV; RSV; HBTEC-ALI; Duplex assay; hMPV; Human metapneumovirus

Human metapneumovirus (hMPV) is a common cause of respiratory infections in children. Many genetic diagnostic assays have been developed, but most detect hMPV regardless of the subgroup. In this study, we developed a real-time RT-PCR assay that can detect and identify the two major subgroups of hMPV...