Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system
Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass sp...
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Published in | Scientific reports Vol. 10; no. 1; p. 13713 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
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England
Nature Publishing Group
13.08.2020
Nature Publishing Group UK |
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Abstract | Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter:BvLz lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter:BvLz lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter:BvLz lines with additional BvLz expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts. |
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AbstractList | Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing
BvLz
under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter:
BvLz
lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter:
BvLz
lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter:
BvLz
lines with additional
BvLz
expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts. Abstract Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter: BvLz lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter: BvLz lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter: BvLz lines with additional BvLz expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts. Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter:BvLz lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter:BvLz lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter:BvLz lines with additional BvLz expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts. Plants represent a safe and cost-effective platform for producing high-value proteins with pharmaceutical properties; however, the ability to accumulate these in commercially viable quantities is challenging. Ideal crops to serve as biofactories would include low-input, fast-growing, high-biomass species such as sugarcane. The objective of this study was to develop an efficient expression system to enable large-scale production of high-value recombinant proteins in sugarcane culms. Bovine lysozyme (BvLz) is a potent broad-spectrum antimicrobial enzyme used in the food, cosmetics and agricultural industries. Here, we report a novel strategy to achieve high-level expression of recombinant proteins using a combinatorial stacked promoter system. We demonstrate this by co-expressing BvLz under the control of multiple constitutive and culm-regulated promoters on separate expression vectors and combinatorial plant transformation. BvLz accumulation reached 1.4% of total soluble protein (TSP) (10.0 mg BvLz/kg culm mass) in stacked multiple promoter:BvLz lines, compared to 0.07% of TSP (0.56 mg/kg) in single promoter:BvLz lines. BvLz accumulation was further boosted to 11.5% of TSP (82.5 mg/kg) through event stacking by re-transforming the stacked promoter:BvLz lines with additional BvLz expression vectors. The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts. |
ArticleNumber | 13713 |
Author | Molina, Joe Damaj, Mona B White, Steven G Barros, Georgia O F Mandadi, Kranthi K Damaj, Bassam B Jifon, John L Woodard, Susan L Vargas-Bautista, Carol Nikolov, Zivko L |
Author_xml | – sequence: 1 givenname: Mona B surname: Damaj fullname: Damaj, Mona B email: mbdamaj@ag.tamu.edu organization: Texas A&M AgriLife Research and Extension Center, 2415 East US Highway 83, Weslaco, TX, 78596, USA. mbdamaj@ag.tamu.edu – sequence: 2 givenname: John L surname: Jifon fullname: Jifon, John L organization: Department of Horticultural Sciences, Texas A&M University, College Station, TX, 77843-2133, USA – sequence: 3 givenname: Susan L surname: Woodard fullname: Woodard, Susan L organization: National Center for Therapeutics Manufacturing, Texas A&M University, 100 Discovery Drive, College Station, TX, 77843-4482, USA – sequence: 4 givenname: Carol surname: Vargas-Bautista fullname: Vargas-Bautista, Carol organization: College of Medicine, Texas A&M University, 8447 Riverside Parkway, Bryan, TX, 77807, USA – sequence: 5 givenname: Georgia O F surname: Barros fullname: Barros, Georgia O F organization: BioSeparation Laboratory, Biological and Agricultural Engineering Department, College Station, TX, 77843-2117, USA – sequence: 6 givenname: Joe surname: Molina fullname: Molina, Joe organization: Texas A&M AgriLife Research and Extension Center, 2415 East US Highway 83, Weslaco, TX, 78596, USA – sequence: 7 givenname: Steven G surname: White fullname: White, Steven G organization: BioSeparation Laboratory, Biological and Agricultural Engineering Department, College Station, TX, 77843-2117, USA – sequence: 8 givenname: Bassam B surname: Damaj fullname: Damaj, Bassam B organization: Innovus Pharmaceuticals, Inc., 8845 Rehco Road, San Diego, CA, 92121, USA – sequence: 9 givenname: Zivko L surname: Nikolov fullname: Nikolov, Zivko L organization: BioSeparation Laboratory, Biological and Agricultural Engineering Department, College Station, TX, 77843-2117, USA – sequence: 10 givenname: Kranthi K surname: Mandadi fullname: Mandadi, Kranthi K email: kkmandadi@tamu.edu, kkmandadi@tamu.edu organization: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, 77843-2132, USA. kkmandadi@tamu.edu |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32792533$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.2135/cropsci2003.1805 10.1007/978-1-60761-765-5_5 10.1016/j.phytochem.2010.01.014 10.1007/BF00020010 10.1371/journal.pone.0130556 10.1111/j.1467-7652.2004.00095.x 10.1111/j.1467-7652.2006.00224.x 10.1111/j.1467-7652.2006.00191.x 10.1007/BF00233416 10.1079/IVP2005643 10.1007/BF01969712 10.1002/btpr.45 10.1111/j.1467-7652.2011.00649.x 10.1007/s00449-012-0878-y 10.1016/j.tibtech.2003.10.002 10.1016/S0958-1669(96)80008-5 10.1016/0965-1748(93)90062-W 10.1080/07388550802046624 10.1172/JCI70266 10.1155/2009/765367 10.1016/j.biotechadv.2009.01.008 10.1186/s13068-017-0850-9 10.1093/nar/26.5.1294 10.1111/j.1467-7652.2012.00686.x 10.1023/A:1009634226797 10.1093/nar/gkp045 10.2135/cropsci1975.0011183X001500030020x 10.1021/jf970156r 10.1007/s12042-011-9080-7 10.1111/pbi.12038 10.1007/s00299-010-0936-3 10.1016/j.virol.2008.10.034 10.3389/fpls.2015.00693 10.1007/978-1-61779-558-9_34 10.1016/j.biotechadv.2017.03.011 10.1111/tpj.13420 10.1007/s00425-010-1138-5 10.1016/S0378-1119(99)00561-2 10.1007/s11248-004-5415-6 10.1016/j.pep.2005.10.026 10.1007/s11103-013-0045-0 10.1002/biot.201500255 10.1007/s12033-014-9758-9 10.1007/s00299-011-1150-7 10.1016/j.tig.2005.04.009 10.1016/S0021-9258(18)90908-7 10.1080/21645698.2018.1553836 10.3390/v11060509 10.1111/j.1467-7652.2010.00550.x 10.1111/pbi.12430 10.1111/j.1467-7652.2010.00507.x 10.1016/0378-1119(91)90614-H 10.1023/A:1009605729268 10.1007/s11032-005-4929-9 10.1007/s12155-012-9288-x 10.4161/psb.1.4.3195 10.1007/BF02668766 10.1111/j.1467-7652.2011.00597.x 10.1073/pnas.93.11.5335 10.3390/plants9010030 10.1371/journal.pone.0013976 10.1111/j.1467-7652.2011.00600.x 10.6090/jarq.45.259 10.1155/2014/136419 10.1155/2013/316304 10.1111/j.1467-7652.2006.00230.x |
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References | SM Brumbley (70530_CR6) 2007; 1297 BL Legendre (70530_CR66) 1975; 15 TH Tai (70530_CR73) 1990; 8 A Gianotti (70530_CR21) 2006; 47 M Ramasamy (70530_CR72) 2018; 9 RB McQualter (70530_CR12) 2005; 3 R Bock (70530_CR32) 2013; 83 JF Buyel (70530_CR44) 2017; 35 HC Sambrook (70530_CR71) 1989 A Wiktorek-Smagur (70530_CR4) 2012 M-L Wang (70530_CR19) 2005; 14 X Zhang (70530_CR50) 2006; 4 D Tokuhara (70530_CR51) 2013; 123 T Mangwende (70530_CR75) 2009; 384 A Kohli (70530_CR56) 2006; 1 EP Rybicki (70530_CR3) 2010; 8 AH Christensen (70530_CR33) 1992; 18 HS Mason (70530_CR49) 1996; 93 P Lakshmanan (70530_CR10) 2005; 41 CP Wilcox (70530_CR31) 1997; 45 IH Greger (70530_CR58) 1998; 26 I Urreta (70530_CR65) 2012 RE Casu (70530_CR76) 2012; 31 M-C Jiang (70530_CR42) 2019; 11 H Marschner (70530_CR68) 1995 C Gatz (70530_CR54) 1996; 7 LA Petrasovits (70530_CR13) 2012; 10 CW Ribeiro (70530_CR22) 2008; 24 A Demain (70530_CR1) 2009; 27 KMJ Butaye (70530_CR55) 2005; 16 K-B Moon (70530_CR40) 2020; 9 BF Chong (70530_CR16) 2010; 71 RA Horlick (70530_CR63) 2000; 243 NR Sahoo (70530_CR27) 2012; 2 F Henrique-Silva (70530_CR20) 2012 MD Harrison (70530_CR23) 2011; 9 T Kadesch (70530_CR60) 1986; 6 RM Twyman (70530_CR5) 2003; 21 FJA Lemos (70530_CR28) 1993; 23 K Duwadi (70530_CR67) 2015; 10 K Maruyama (70530_CR53) 2017; 89 G Beyene (70530_CR38) 2011; 30 J Zischewski (70530_CR43) 2016; 11 70530_CR29 MD Harrison (70530_CR24) 2014; 56 J Sambrook (70530_CR74) 2001 L Borghi (70530_CR52) 2010 I Ingelbrecht (70530_CR59) 1991; 109 L Wu (70530_CR18) 2007; 5 MP Purnell (70530_CR14) 2007; 5 K Tilbrook (70530_CR15) 2011; 9 JM Ruijter (70530_CR77) 2009; 37 P Jolles (70530_CR26) 1984; 259 E Egelkrout (70530_CR64) 2013; 6 F Sainsbury (70530_CR45) 2010; 5 HM Davies (70530_CR69) 2010; 8 SR Mudge (70530_CR17) 2013; 11 DJ Anderson (70530_CR11) 2011; 4 70530_CR39 M Merlin (70530_CR41) 2014; 2014 MB Damaj (70530_CR78) 2009; 2009 M Yang (70530_CR36) 2003; 43 70530_CR37 ME Digan (70530_CR80) 1989; 7 M Gallo-Meagher (70530_CR9) 1993; 12 MB Damaj (70530_CR35) 2010; 231 SB Maqbool (70530_CR61) 1999; 5 GOF Barros (70530_CR30) 2013; 36 70530_CR79 S Ando (70530_CR7) 2011; 45 A Porceddu (70530_CR47) 1999; 5 EA Uvarova (70530_CR48) 2013; 2013 S-J Gao (70530_CR34) 2017; 10 KE Shearwin (70530_CR62) 2005; 21 AH Christensen (70530_CR70) 1996; 5 P Basaran (70530_CR2) 2008; 28 S Matsuoka (70530_CR8) 2014; 2014 S Rajeev Kumar (70530_CR57) 2015; 6 H Palaniswamy (70530_CR25) 2016; 14 H Lai (70530_CR46) 2012; 10 |
References_xml | – volume: 43 start-page: 1805 year: 2003 ident: 70530_CR36 publication-title: Crop Sci. doi: 10.2135/cropsci2003.1805 contributor: fullname: M Yang – start-page: 65 volume-title: Plant Developmental Biology year: 2010 ident: 70530_CR52 doi: 10.1007/978-1-60761-765-5_5 contributor: fullname: L Borghi – volume: 71 start-page: 736 year: 2010 ident: 70530_CR16 publication-title: Phytochemistry doi: 10.1016/j.phytochem.2010.01.014 contributor: fullname: BF Chong – volume: 18 start-page: 675 year: 1992 ident: 70530_CR33 publication-title: Plant Mol. Biol. doi: 10.1007/BF00020010 contributor: fullname: AH Christensen – volume: 10 start-page: e0130556 year: 2015 ident: 70530_CR67 publication-title: PLoS ONE doi: 10.1371/journal.pone.0130556 contributor: fullname: K Duwadi – volume: 3 start-page: 29 year: 2005 ident: 70530_CR12 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2004.00095.x contributor: fullname: RB McQualter – volume: 5 start-page: 109 year: 2007 ident: 70530_CR18 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2006.00224.x contributor: fullname: L Wu – volume: 4 start-page: 419 year: 2006 ident: 70530_CR50 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2006.00191.x contributor: fullname: X Zhang – start-page: 305 volume-title: Transgenic Plants-Advances and Limitations year: 2012 ident: 70530_CR65 contributor: fullname: I Urreta – volume: 12 start-page: 666 year: 1993 ident: 70530_CR9 publication-title: Plant Cell Rep. doi: 10.1007/BF00233416 contributor: fullname: M Gallo-Meagher – volume: 41 start-page: 345 year: 2005 ident: 70530_CR10 publication-title: Vitro Cell. Dev. Biol. Plant doi: 10.1079/IVP2005643 contributor: fullname: P Lakshmanan – volume: 6 start-page: 2593 year: 1986 ident: 70530_CR60 publication-title: Mol. Cell. Biol. contributor: fullname: T Kadesch – volume: 5 start-page: 213 year: 1996 ident: 70530_CR70 publication-title: Transgenic Res. doi: 10.1007/BF01969712 contributor: fullname: AH Christensen – volume: 24 start-page: 1060 year: 2008 ident: 70530_CR22 publication-title: Biotechnol. Prog. doi: 10.1002/btpr.45 contributor: fullname: CW Ribeiro – ident: 70530_CR39 – volume: 10 start-page: 95 year: 2012 ident: 70530_CR46 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2011.00649.x contributor: fullname: H Lai – volume: 36 start-page: 1407 year: 2013 ident: 70530_CR30 publication-title: Bioprocess Biosyst. Eng. doi: 10.1007/s00449-012-0878-y contributor: fullname: GOF Barros – volume: 1297 start-page: 5 year: 2007 ident: 70530_CR6 publication-title: Int. Sugar J. contributor: fullname: SM Brumbley – volume: 21 start-page: 570 year: 2003 ident: 70530_CR5 publication-title: Trends Biotechnol. doi: 10.1016/j.tibtech.2003.10.002 contributor: fullname: RM Twyman – volume: 7 start-page: 168 year: 1996 ident: 70530_CR54 publication-title: Curr. Opin. Biotechnol. doi: 10.1016/S0958-1669(96)80008-5 contributor: fullname: C Gatz – volume: 23 start-page: 533 year: 1993 ident: 70530_CR28 publication-title: Insect Biochem. Mol. Biol. doi: 10.1016/0965-1748(93)90062-W contributor: fullname: FJA Lemos – volume: 28 start-page: 153 year: 2008 ident: 70530_CR2 publication-title: Crit. Rev. Biotechnol. doi: 10.1080/07388550802046624 contributor: fullname: P Basaran – volume: 123 start-page: 3829 year: 2013 ident: 70530_CR51 publication-title: J. Clin. Investig. doi: 10.1172/JCI70266 contributor: fullname: D Tokuhara – volume: 2009 start-page: 13 year: 2009 ident: 70530_CR78 publication-title: Int. J. Plant Genom. doi: 10.1155/2009/765367 contributor: fullname: MB Damaj – volume: 27 start-page: 297 year: 2009 ident: 70530_CR1 publication-title: Biotechnol. Adv. doi: 10.1016/j.biotechadv.2009.01.008 contributor: fullname: A Demain – volume: 10 start-page: 172 year: 2017 ident: 70530_CR34 publication-title: Biotechnol. Biofuels doi: 10.1186/s13068-017-0850-9 contributor: fullname: S-J Gao – volume: 26 start-page: 1294 year: 1998 ident: 70530_CR58 publication-title: Nucleic Acids Res. doi: 10.1093/nar/26.5.1294 contributor: fullname: IH Greger – volume: 10 start-page: 569 year: 2012 ident: 70530_CR13 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2012.00686.x contributor: fullname: LA Petrasovits – volume: 5 start-page: 471 year: 1999 ident: 70530_CR61 publication-title: Mol. Breed. doi: 10.1023/A:1009634226797 contributor: fullname: SB Maqbool – volume: 37 start-page: e45 year: 2009 ident: 70530_CR77 publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkp045 contributor: fullname: JM Ruijter – volume: 15 start-page: 349 year: 1975 ident: 70530_CR66 publication-title: Crop Sci. doi: 10.2135/cropsci1975.0011183X001500030020x contributor: fullname: BL Legendre – volume: 45 start-page: 2793 year: 1997 ident: 70530_CR31 publication-title: J. Agric. Food Chem. doi: 10.1021/jf970156r contributor: fullname: CP Wilcox – volume: 4 start-page: 170 year: 2011 ident: 70530_CR11 publication-title: Trop. Plant Biol. doi: 10.1007/s12042-011-9080-7 contributor: fullname: DJ Anderson – volume: 11 start-page: 502 year: 2013 ident: 70530_CR17 publication-title: Plant Biotechnol. J. doi: 10.1111/pbi.12038 contributor: fullname: SR Mudge – volume: 30 start-page: 13 year: 2011 ident: 70530_CR38 publication-title: Plant Cell Rep. doi: 10.1007/s00299-010-0936-3 contributor: fullname: G Beyene – volume: 384 start-page: 38 year: 2009 ident: 70530_CR75 publication-title: Virology doi: 10.1016/j.virol.2008.10.034 contributor: fullname: T Mangwende – volume: 6 start-page: 693 year: 2015 ident: 70530_CR57 publication-title: Front. Plant Sci. doi: 10.3389/fpls.2015.00693 contributor: fullname: S Rajeev Kumar – volume: 2014 year: 2014 ident: 70530_CR8 publication-title: Adv. Bot. contributor: fullname: S Matsuoka – start-page: 437 volume-title: Transgenic Plants year: 2012 ident: 70530_CR20 doi: 10.1007/978-1-61779-558-9_34 contributor: fullname: F Henrique-Silva – volume: 35 start-page: 458 year: 2017 ident: 70530_CR44 publication-title: Biotechnol. Adv. doi: 10.1016/j.biotechadv.2017.03.011 contributor: fullname: JF Buyel – volume: 89 start-page: 671 year: 2017 ident: 70530_CR53 publication-title: Plant J. doi: 10.1111/tpj.13420 contributor: fullname: K Maruyama – volume-title: Molecular Cloning: A Laboratory Manual year: 1989 ident: 70530_CR71 contributor: fullname: HC Sambrook – volume: 231 start-page: 1439 year: 2010 ident: 70530_CR35 publication-title: Planta doi: 10.1007/s00425-010-1138-5 contributor: fullname: MB Damaj – volume: 243 start-page: 187 year: 2000 ident: 70530_CR63 publication-title: Gene doi: 10.1016/S0378-1119(99)00561-2 contributor: fullname: RA Horlick – volume: 14 start-page: 167 year: 2005 ident: 70530_CR19 publication-title: Transgenic Res. doi: 10.1007/s11248-004-5415-6 contributor: fullname: M-L Wang – volume: 47 start-page: 483 year: 2006 ident: 70530_CR21 publication-title: Protein Expr. Purif. doi: 10.1016/j.pep.2005.10.026 contributor: fullname: A Gianotti – start-page: 63 volume-title: Transgenic Plants-Advances and Limitations year: 2012 ident: 70530_CR4 contributor: fullname: A Wiktorek-Smagur – volume: 83 start-page: 21 year: 2013 ident: 70530_CR32 publication-title: Plant Mol. Biol. doi: 10.1007/s11103-013-0045-0 contributor: fullname: R Bock – volume: 11 start-page: 107 year: 2016 ident: 70530_CR43 publication-title: Biotechnol. J. doi: 10.1002/biot.201500255 contributor: fullname: J Zischewski – volume: 56 start-page: 795 year: 2014 ident: 70530_CR24 publication-title: Mol. Biotechnol. doi: 10.1007/s12033-014-9758-9 contributor: fullname: MD Harrison – ident: 70530_CR79 – volume: 31 start-page: 167 year: 2012 ident: 70530_CR76 publication-title: Plant Cell Rep. doi: 10.1007/s00299-011-1150-7 contributor: fullname: RE Casu – volume: 21 start-page: 339 year: 2005 ident: 70530_CR62 publication-title: Trends Genet. doi: 10.1016/j.tig.2005.04.009 contributor: fullname: KE Shearwin – volume: 259 start-page: 11617 year: 1984 ident: 70530_CR26 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)90908-7 contributor: fullname: P Jolles – ident: 70530_CR37 – volume: 9 start-page: 211 year: 2018 ident: 70530_CR72 publication-title: GM Crops Food doi: 10.1080/21645698.2018.1553836 contributor: fullname: M Ramasamy – volume: 11 start-page: 509 year: 2019 ident: 70530_CR42 publication-title: Viruses doi: 10.3390/v11060509 contributor: fullname: M-C Jiang – volume: 8 start-page: 845 year: 2010 ident: 70530_CR69 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2010.00550.x contributor: fullname: HM Davies – volume: 7 start-page: 160 year: 1989 ident: 70530_CR80 publication-title: Biotechnology contributor: fullname: ME Digan – volume: 2 start-page: 347 year: 2012 ident: 70530_CR27 publication-title: J. Anim. Sci. Adv. contributor: fullname: NR Sahoo – volume: 14 start-page: 791 year: 2016 ident: 70530_CR25 publication-title: Plant Biotechnol. J. doi: 10.1111/pbi.12430 contributor: fullname: H Palaniswamy – volume: 8 start-page: 620 year: 2010 ident: 70530_CR3 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2010.00507.x contributor: fullname: EP Rybicki – volume: 109 start-page: 239 year: 1991 ident: 70530_CR59 publication-title: Gene doi: 10.1016/0378-1119(91)90614-H contributor: fullname: I Ingelbrecht – volume: 5 start-page: 553 year: 1999 ident: 70530_CR47 publication-title: Mol. Breed. doi: 10.1023/A:1009605729268 contributor: fullname: A Porceddu – volume: 16 start-page: 79 year: 2005 ident: 70530_CR55 publication-title: Mol. Breed. doi: 10.1007/s11032-005-4929-9 contributor: fullname: KMJ Butaye – volume: 6 start-page: 699 year: 2013 ident: 70530_CR64 publication-title: BioEnergy Res. doi: 10.1007/s12155-012-9288-x contributor: fullname: E Egelkrout – volume: 1 start-page: 185 year: 2006 ident: 70530_CR56 publication-title: Plant Signal. Behav. doi: 10.4161/psb.1.4.3195 contributor: fullname: A Kohli – ident: 70530_CR29 – volume: 8 start-page: 297 year: 1990 ident: 70530_CR73 publication-title: Plant Mol. Biol. Report. doi: 10.1007/BF02668766 contributor: fullname: TH Tai – volume: 9 start-page: 884 year: 2011 ident: 70530_CR23 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2011.00597.x contributor: fullname: MD Harrison – volume: 93 start-page: 5335 year: 1996 ident: 70530_CR49 publication-title: Proc. Natl. Acad. Sci. doi: 10.1073/pnas.93.11.5335 contributor: fullname: HS Mason – volume-title: Mineral Nutrition of Higher Plants year: 1995 ident: 70530_CR68 contributor: fullname: H Marschner – volume: 9 start-page: 30 year: 2020 ident: 70530_CR40 publication-title: Plants doi: 10.3390/plants9010030 contributor: fullname: K-B Moon – volume: 5 start-page: e13976 year: 2010 ident: 70530_CR45 publication-title: PLoS ONE doi: 10.1371/journal.pone.0013976 contributor: fullname: F Sainsbury – volume: 9 start-page: 958 year: 2011 ident: 70530_CR15 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2011.00600.x contributor: fullname: K Tilbrook – volume-title: Molecular Cloning: A Laboratory Manual year: 2001 ident: 70530_CR74 contributor: fullname: J Sambrook – volume: 45 start-page: 259 year: 2011 ident: 70530_CR7 publication-title: Jpn. Agric. Res. Q. doi: 10.6090/jarq.45.259 contributor: fullname: S Ando – volume: 2014 start-page: 1 year: 2014 ident: 70530_CR41 publication-title: BioMed Res. Int. doi: 10.1155/2014/136419 contributor: fullname: M Merlin – volume: 2013 start-page: 1 year: 2013 ident: 70530_CR48 publication-title: BioMed Res. Int. doi: 10.1155/2013/316304 contributor: fullname: EA Uvarova – volume: 5 start-page: 173 year: 2007 ident: 70530_CR14 publication-title: Plant Biotechnol. J. doi: 10.1111/j.1467-7652.2006.00230.x contributor: fullname: MP Purnell |
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SubjectTerms | Animals Cattle Cosmetics Expression vectors Food industry Genetic transformation Lysozyme Muramidase - genetics Muramidase - isolation & purification Muramidase - metabolism Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Promoter Regions, Genetic Proteins Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Saccharum - genetics Saccharum - growth & development Stacking Sugarcane Transformation, Genetic |
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Title | Unprecedented enhancement of recombinant protein production in sugarcane culms using a combinatorial promoter stacking system |
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