Molecular characterization of mosquitocidal Bacillus sphaericus isolated from Tamil Nadu, India

• Published in: Acta tropica Vol. 127; no. 3; pp. 158 - 164
• Main Authors: Immanual Gilwax Prabhu, D., Gowri Sankar, S., Thirumalai Vasan, P., Sobana Piriya, P., Karpanai Selvan, B., John Vennison, S.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2013
• Subjects: Animals; Anopheles stephensi; Bacillus - classification; Bacillus - isolation & purification; Bacillus - metabolism; Bacillus sphaericus; Bacterial Toxins - genetics; Bacterial Toxins - metabolism; Binary toxin; bioassays; Culex - microbiology; Culex quinquefasciatus; Dendrogram; Gene Expression Regulation, Bacterial; genes; genetic heterogeneity; India; insect larvae; Larva - microbiology; Mosquito Control - methods; Mosquitocidal toxin; Multiplex PCR; Pest Control, Biological - methods; Phylogeny; polymerase chain reaction; Polymerase Chain Reaction - methods; REP-PCR; screening; soil; toxicity; India; India, Tamil Nadu; Multiplex PCR; Binary toxin; Mosquitocidal toxin; Dendrogram; REP-PCR; Bacillus sphaericus

Forty-two Bacillus sphaericus strains were isolated from soil around Tamil Nadu, India. Phylogenetic relationship and presence of mosquito larvicidal genes in the isolates were analysed by REP-PCR and multiplex PCR respectively. Three isolates showed high level of toxicity and they were grouped dist...