Characterizing well-differentiated culture of primary human nasal epithelial cells for use in wound healing assays

• Published in: Laboratory investigation Vol. 98; no. 11; pp. 1478 - 1486
• Main Authors: Schagen, Johanna, Sly, Peter D., Fantino, Emmanuelle
• Format: Journal Article
• Language: English
• Published: New York Elsevier Inc 01.11.2018; Nature Publishing Group US; Nature Publishing Group
• Subjects: 13/106; 13/21; 14/1; 14/19; 631/1647/767/2201; 692/699/1785; Cell culture; Cell Differentiation; Cytokines - metabolism; Epidermal growth factor receptors; Epithelial cells; Epithelium; ErbB Receptors - antagonists & inhibitors; Erlotinib Hydrochloride; Female; Humans; Inhibition; Laboratory Medicine; Maintenance; Male; Medicine; Medicine & Public Health; Nasal Mucosa - cytology; Pain perception; Pathology; Primary Cell Culture; Respiratory tract; Wound Healing

The nasal epithelium is the initial contact between the external environment and the respiratory tract and how it responds to noxious stimuli and repairs epithelial damage is important. Growing airway epithelial cells in culture at air-liquid interface allows for a physiologically relevant model of...