A novel fed-batch strategy enhances lipid and astaxanthin productivity without compromising biomass of Chromochloris zofingiensis
[Display omitted] •Fed-batch strategy increased 41.5% lipid productivity without compromising biomass.•Carbon dependent kinetics under stress conferred cells with a higher biomass.•Central carbon metabolism offered energy and carbon availability for lipid synthesis.•Sufficient precursor and active s...
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| Published in | Bioresource technology Vol. 308; p. 123306 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier Ltd
01.07.2020
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| Subjects | |
| Online Access | Get full text |
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| Abstract | [Display omitted]
•Fed-batch strategy increased 41.5% lipid productivity without compromising biomass.•Carbon dependent kinetics under stress conferred cells with a higher biomass.•Central carbon metabolism offered energy and carbon availability for lipid synthesis.•Sufficient precursor and active synthetic pathway promoted lipid content.•Fed-batch strategy was attractive by understanding lipid metabolism in detail.
To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L−1 d−1, respectively. Biomass productivity at 1084.3 mg L−1 d−1 was comparable with that under suitable condition. Then 13C tracer-based metabolic flux analysis (13C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, 13C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation. |
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| AbstractList | To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L-1 d-1, respectively. Biomass productivity at 1084.3 mg L-1 d-1 was comparable with that under suitable condition. Then 13C tracer-based metabolic flux analysis (13C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, 13C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation.To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L-1 d-1, respectively. Biomass productivity at 1084.3 mg L-1 d-1 was comparable with that under suitable condition. Then 13C tracer-based metabolic flux analysis (13C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, 13C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation. [Display omitted] •Fed-batch strategy increased 41.5% lipid productivity without compromising biomass.•Carbon dependent kinetics under stress conferred cells with a higher biomass.•Central carbon metabolism offered energy and carbon availability for lipid synthesis.•Sufficient precursor and active synthetic pathway promoted lipid content.•Fed-batch strategy was attractive by understanding lipid metabolism in detail. To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L−1 d−1, respectively. Biomass productivity at 1084.3 mg L−1 d−1 was comparable with that under suitable condition. Then 13C tracer-based metabolic flux analysis (13C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, 13C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation. To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L d , respectively. Biomass productivity at 1084.3 mg L d was comparable with that under suitable condition. Then C tracer-based metabolic flux analysis ( C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation. To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen stress was applied under excess light to elevate intracellular carbon availability and metabolic activity of Chromochloris zofingiensis. Results suggested that fed-batch strategy proposed could increase lipid and astaxanthin productivity to 457.1 and 2.0 mg L⁻¹ d⁻¹, respectively. Biomass productivity at 1084.3 mg L⁻¹ d⁻¹ was comparable with that under suitable condition. Then ¹³C tracer-based metabolic flux analysis (¹³C-MFA) demonstrated that central carbon metabolism provided ATP, NADPH and carbon availability for lipid biosynthesis during the strategy. In combination with targeted metabolite analysis, ¹³C-MFA revealed that the strategy improved precursor content for lipid biosynthesis and elevated path rate to synthesize C16:0 and C18:0. The enhanced lipid content potentially accounted for the high biomass productivity. Therefore, comprehensively understanding relationships between carbon availability and carbon conversion could precisely design strategy for productivity improvements during cultivation. |
| ArticleNumber | 123306 |
| Author | Chen, Feng Lao, Yongmin Sun, Han Ren, Yuanyuan Li, Xiaojie |
| Author_xml | – sequence: 1 givenname: Han surname: Sun fullname: Sun, Han organization: Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China – sequence: 2 givenname: Yuanyuan surname: Ren fullname: Ren, Yuanyuan organization: Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China – sequence: 3 givenname: Yongmin surname: Lao fullname: Lao, Yongmin organization: Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China – sequence: 4 givenname: Xiaojie surname: Li fullname: Li, Xiaojie organization: Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China – sequence: 5 givenname: Feng surname: Chen fullname: Chen, Feng email: sfchen@szu.edu.cn organization: Institute for Advanced Study, Shenzhen University, Shenzhen 518060, China |
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| Keywords | Carbon conversion Fatty acid metabolism Carbon availability Fed-batch culture Astaxanthin |
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•Fed-batch strategy increased 41.5% lipid productivity without compromising biomass.•Carbon dependent kinetics under stress conferred cells... To improve lipid and astaxanthin productivity without compromising biomass during the whole cultivation period, carbon-dependent kinetics involving nitrogen... |
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| SubjectTerms | adenosine triphosphate Astaxanthin biochemical pathways Biomass biomass production biosynthesis carbon Carbon availability Carbon conversion carbon metabolism Fatty acid metabolism Fed-batch culture lipid content Lipids metabolic flux analysis metabolites NADP (coenzyme) Nitrogen Xanthophylls |
| Title | A novel fed-batch strategy enhances lipid and astaxanthin productivity without compromising biomass of Chromochloris zofingiensis |
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