Low Initial Tau Phosphorylation in Human Brain Biopsy Samples

• Published in: Neurobiology of aging Vol. 18; no. 5; pp. 475 - 481
• Main Authors: Song, J, Combs, C.K, Pilcher, W.H, Song, L.Y, Utal, A.K, Coleman, P.D
• Format: Journal Article
• Language: English
• Published: London Elsevier Inc 01.09.1997; Elsevier Science
• Subjects: Adolescent; Adult; Alzheimer; Animals; Biological and medical sciences; Biopsy; Blotting, Western; Brain Chemistry - physiology; Cell Death; Child; Culture Media; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases; Epilepsy - metabolism; Epilepsy - surgery; Human brain biopsy; Humans; In Vitro Techniques; Medical sciences; Middle Aged; Neocortex - metabolism; Nerve Tissue Proteins - metabolism; Neurofilament Proteins - metabolism; Neurology; Paired helical filaments; Phosphorylation; Protein phosphorylation; Rats; Rats, Sprague-Dawley; Tau protein; tau Proteins - metabolism; Human brain biopsy; Tau protein; Protein phosphorylation; Alzheimer; Paired helical filaments; Human; Nervous system diseases; Cerebral cortex; Phosphorylation; Rat; Alzheimer disease; Paired helical filament; Rodentia; Central nervous system; In vitro; Cerebral disorder; Vertebrata; Mammalia; Biopsy; Central nervous system disease; Degenerative disease; Brain (vertebrata)

A rapid reversible tau phosphorylation at Ser 396/404 was observed in adult human cortical biopsy tissue and rat primary cortical cell cultures. Tau phosphorylation increased usually during the first 20–30 min in phosphate-buffered saline, followed by a decrease. The time course of tau phosphorylati...